Lipase-nanoporous gold biocomposite modified electrode for reliable detection of triglycerides.
Lipase-nanoporous gold biocomposite modified electrode for reliable detection of triglycerides.
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DOI：
10.1016/j.bios.2013.09.040
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发表时间：
2014-03
影响因子：
12.6
通讯作者：
Chao Wu;Xueying Liu;Yufei Li;Xiaoyu Du;Xia Wang;P. Xu
中科院分区：
文献类型：
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作者：
Chao Wu;Xueying Liu;Yufei Li;Xiaoyu Du;Xia Wang;P. Xu
对于甘油三酯生物传感器的设计，蛋白质固定化是必要的，以创建酶和电极之间的界面。在这项研究中，脂酶-纳米孔金（NPG）生物复合材料（标记为脂肪酶/NPG/GCE）修饰了玻璃碳电极（GCE）。由于NPG具有高导电性、多孔性和生物相容性的三维结构，因此适合用于酶的固定化。在循环伏安实验中，脂肪酶/NPG/GCE生物电极在磷酸盐缓冲溶液中表现出表面限制反应。当三丁酸甘油酯浓度为50 ~ 250 mg dl - 1，橄榄油浓度为10 ~ 200 mg dl - 1时，得到线性响应。三丁酸甘油酯的表观Michaelis-Menten常数为10.67 mg dl - 1，检出限为2.68 mg dl - 1。此外，脂肪酶/NPG/GCE生物电极对尿素、葡萄糖、胆固醇和尿酸具有较强的抗干扰能力，并且具有较长的保质期。对于人血清中甘油三酯的检测，脂肪酶/NPG/GCE生物电极测定值与全自动生化分析仪测定值吻合较好。这些特性以及较长的自我寿命使脂肪酶/NPG/GCE生物电极成为构建甘油三酯生物传感器的理想选择。
For triglycerides biosensor design, protein immobilization is necessary to create the interface between the enzyme and the electrode. In this study, a glassy carbon electrode (GCE) was modified with lipase-nanoporous gold (NPG) biocomposite (denoted as lipase/NPG/GCE). Due to highly conductive, porous, and biocompatible three-dimensional structure, NPG is suitable for enzyme immobilization. In cyclic voltammetry experiments, the lipase/NPG/GCE bioelectrode displayed surface-confined reaction in a phosphate buffer solution. Linear responses were obtained for tributyrin concentrations ranging from 50 to 250 mg dl−1and olive oil concentrations ranging from 10 to 200 mg dl−1. The value of apparent Michaelis–Menten constant for tributyrin was 10.67 mg dl−1and the detection limit was 2.68 mg dl−1. Further, the lipase/NPG/GCE bioelectrode had strong anti-interference ability against urea, glucose, cholesterol, and uric acid as well as a long shelf-life. For the detection of triglycerides in human serum, the values given by the lipase/NPG/GCE bioelectrode were in good agreement with those of an automatic biochemical analyzer. These properties along with a long self-life make the lipase/NPG/GCE bioelectrode an excellent choice for the construction of triglycerides biosensor.