* Hierarchically Structured Electrospun Scaffolds with Chemically Conjugated Growth Factor for Ligament Tissue Engineering.
* Hierarchically Structured Electrospun Scaffolds with Chemically Conjugated Growth Factor for Ligament Tissue Engineering.
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DOI：
10.1089/ten.tea.2016.0480
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发表时间：
2017-08
影响因子：
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通讯作者：
H. Pauly;B. Sathy;Dinorath Olvera;H. McCarthy;D. Kelly;K. Popat;N. Dunne;T. H. Haut Donahue
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文献类型：
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作者：
H. Pauly;B. Sathy;Dinorath Olvera;H. McCarthy;D. Kelly;K. Popat;N. Dunne;T. H. Haut Donahue
膝关节前交叉韧带（ACL）对关节功能至关重要，通常在运动损伤或车祸中断裂。由于缺乏内在的愈合能力以及同种异体移植物和自体移植物的缺点，需要组织工程化ACL置换。我们的小组以前已经使用电纺聚己内酯纳米纤维的对齐片材来开发纵向对齐的纳米纤维的实心圆柱形束。我们已经表明，这些ACL束支持细胞增殖和伸长，并且其层次结构和材料性质与天然人ACL相似。联合收割机可以将多个ACL束组合以创建试图模拟ACL的宏观结构的支架。本研究的目的是利用结缔组织生长因子（CTGF）偶联的胶原纤维束构建一种用于韧带组织工程的生物活性支架，并评价骨髓间充质干细胞（MSCs）在该支架上的体内外行为。CTGF被固定在单个纤维束或由多个纤维束组成的支架的表面上。使用X-射线光电子能谱、测定和免疫荧光染色评估缀合效率和缀合的CTGF的释放。用MSC接种支架并在体外维持7天（单独的CTGF束）、体外维持21天（20个CTGF束的按比例放大的支架）或体内维持6周（4个CTGF束的小支架），并与非CTGF缀合的对照支架相比评估韧带特异性组织形成。结果表明，CTGF结合促进细胞增殖和韧带特异性组织形成在体外和体内。结果表明，与CTGF缀合的分级静电纺丝纤维束是用于ACL组织工程的可扩展的和生物活性的支架。
The anterior cruciate ligament (ACL) of the knee is vital for proper joint function and is commonly ruptured during sports injuries or car accidents. Due to a lack of intrinsic healing capacity and drawbacks with allografts and autografts, there is a need for a tissue-engineered ACL replacement. Our group has previously used aligned sheets of electrospun polycaprolactone nanofibers to develop solid cylindrical bundles of longitudinally aligned nanofibers. We have shown that these nanofiber bundles support cell proliferation and elongation and the hierarchical structure and material properties are similar to the native human ACL. It is possible to combine multiple nanofiber bundles to create a scaffold that attempts to mimic the macroscale structure of the ACL. The goal of this work was to develop a hierarchical bioactive scaffold for ligament tissue engineering using connective tissue growth factor (CTGF)-conjugated nanofiber bundles and evaluate the behavior of mesenchymal stem cells (MSCs) on these scaffolds in vitro and in vivo. CTGF was immobilized onto the surface of individual nanofiber bundles or scaffolds consisting of multiple nanofiber bundles. The conjugation efficiency and the release of conjugated CTGF were assessed using X-ray photoelectron spectroscopy, assays, and immunofluorescence staining. Scaffolds were seeded with MSCs and maintained in vitro for 7 days (individual nanofiber bundles), in vitro for 21 days (scaled-up scaffolds of 20 nanofiber bundles), or in vivo for 6 weeks (small scaffolds of 4 nanofiber bundles), and ligament-specific tissue formation was assessed in comparison to non-CTGF-conjugated control scaffolds. Results showed that CTGF conjugation encouraged cell proliferation and ligament-specific tissue formation in vitro and in vivo. The results suggest that hierarchical electrospun nanofiber bundles conjugated with CTGF are a scalable and bioactive scaffold for ACL tissue engineering.