The effects of spinal cord transection and intracisternal 6‐hydroxydopamine on phenylethanolamine‐N‐methyl transferase (PNMT) activity in rat brain stem and spinal cord
The effects of spinal cord transection and intracisternal 6‐hydroxydopamine on phenylethanolamine‐N‐methyl transferase (PNMT) activity in rat brain stem and spinal cord
复制标题
脊髓横断和脑池内注射6-羟基多巴胺对大鼠脑干和脊髓苯乙醇胺-N-甲基转移酶（PNMT）活性的影响
DOI：
10.1111/j.1471-4159.1976.tb01524.x
复制
发表时间：
1976
影响因子：
4.7
通讯作者：
I. Kopin
中科院分区：
文献类型：
--
作者：
J. Reid;J. Zivin;I. Kopin
最近的特定质量碎片图（KOSLOW & SCHLUMPF. 1974）和放射性测定技术（摩尔& PHILLIPSON，1975; REID等人，1975）已经证实下丘脑中存在肾上腺素。大鼠和兔的脑干和脊髓。苯乙醇胺-N-甲基转移酶（PNMT）（EC 2.1.1.28）也可以在脑中得到证实（CIARANELLO等人，1969; MCGEER & MCGEER，1964; POHRBCKY等人，1969年）。免疫组织化学研究表明，PNMT存在于神经元的细胞体、轴突和轴突中，所述神经元起源于脑干的外侧区域，并且上升至下丘脑或下降至脊髓的灰质中，特别是在侧胸柱中（H-KFELT等人，1974年）。这种分布得到了脑中PNMT活性的研究的支持（SAAVESTIVE et al. 1974）和脊髓。似乎肾上腺素可能是中枢神经系统中的神经递质，并且其可能在觉醒的调节中起作用（FUXE等人，2005）。1974）和包括血压在内的自主活动（BOLME等，1974年）。在本研究中，我们研究了脊髓横断对近端和远端脊髓节段PNMT活性的影响。我们还检查了脑池内6-羟基多巴胺（6-OHDA）对脑干和脊髓PNMT活性的影响，作为含中枢肾上腺素的神经元对该化合物神经毒性作用的易感性的指示（THOENEN & TRANZER，1968; URETSKY & IVERSM，1969）。
RECENTLY specific mass fragmentographic (KOSLOW & SCHLUMPF. 1974) and radio-metric assay techniques (MOORE & PHILLIPSON, 1975; REID et al., 1975) have confirmed the presence of epinephrine in hypothalamus. brain stem and spinal cord of rats and rabbits. The enzyme phenylethanolamine-N-methyl transferase (PNMT) (EC 2.1.1.28) can also be demonstrated in brain (CIARANELLO et al., 1969; MCGEER & MCGEER, 1964; POHRBCKY et al., 1969). Immunohistochemical studies suggest that PNMT is present in cell bodies, axons and tcrrninals of neurones which originate in the lateral areas of the brain stem and either ascend to the hypothalamus or descend to terminate in the grey mattcr of the spinal cord, particularly in the lateral thoracic column (H~KFELT et a!., 1974). This distribution is supported by studies mapping PNMT activity in brain (SAAVEDRA et al.. 1974) and spinal cord. It appears likely that epinephrine is a neurotransmitter in the central nervous system and it may play a role in the regulation of wakefulness (FUXE ei uL. 1974) and aulonomic activity including blood pressure (BOLME et al., 1974). In the present study we have investigated the effects of spinal cord transcction on PNMT activity in proximal and distal segments of cord. We have also examined the effect of intracisternal 6-hydroxydopamine (6-OHDA) on brain stem and spinal cord PNMT activity as an indication of the susceptibility of central epinephrine containing neurones to the neurotoxic effects of this compound (THOENEN & TRANZER, 1968; URETSKY & IVERSM, 1969).