CYTOSKELETAL CHANGES INDUCED IN HEP-2 CELLS BY THE CYTO-TOXIC NECROTIZING FACTOR OF ESCHERICHIA-COLI
CYTOSKELETAL CHANGES INDUCED IN HEP-2 CELLS BY THE CYTO-TOXIC NECROTIZING FACTOR OF ESCHERICHIA-COLI
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DOI：
10.1016/0041-0101(88)90203-6
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发表时间：
1988-01-01
期刊：
影响因子：
2.8
通讯作者：
DONELLI, G
中科院分区：
文献类型：
--
作者：
FIORENTINI, C;ARANCIA, G;DONELLI, G
应用荧光显微镜和扫描电镜观察了大肠杆菌细胞毒坏死因子对HEp-2细胞的作用。这种细胞毒素，已知在几种细胞系中诱导形成巨大的变核细胞，引起肌动蛋白和微管蛋白组织的变化。细胞边缘出现膜皱褶，细胞体上有许多粗的肌动蛋白束，表明该因子促进细胞铺展;这可能干扰胞质分裂，最终导致形成非常大的扁平多核细胞。此外，在处理的细胞中观察到的核分割似乎与肌动蛋白在核周区域的重排和微管蛋白束在核裂附近的存在有关。尽管主要靶点尚不清楚，但这些发现表明细胞骨架受到影响，导致该因子诱导的多核化过程。
The effect of the cytotoxic necrotizing factor of Escherichia coli on HEp-2 cells was studied by fluorescence and scanning electron microscopy. This cytotoxin, known for inducing the formation of giant mutinucleated cells in several cell lines, caused changes in actin and tubulin organization. The presence of membrane ruffles at the cell border and of numerous thick bundles of actin crossing the cell body, suggests that the factor promotes cell spreading; this probably interferes with cytokinesis, ultimately leading to the formation of very large flattened multinucleated cells. Moreover, the nuclear segmentation observed in treated cells seem to be associated with a rearrangement of actin in the perinuclear region and with the presence of tubulin bundles in proximity to nuclear clefts. Although the primary target is still unknown, these findings suggest that the cytoskeleton is affected accounting for the multinucleation process induced by the factor.