Cell division angle regulates the tissue mechanics and tunes the amount of cerebellar folding.
Cell division angle regulates the tissue mechanics and tunes the amount of cerebellar folding.
复制标题
细胞分裂角度调节组织力学并调节小脑折叠的量。
DOI：
10.1101/2023.07.21.549165
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Lawton,AndrewK
中科院分区：
文献类型：
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作者：
Cook,AmberG;Bishop,TaylorV;Crowe,HannahR;Stevens,Daniel;Reine,Lauren;Joyner,AlexandraL;Lawton,AndrewK
模型提出，神经组织的折叠量由组织层之间的差异膨胀程度决定，波长由外层的厚度设置。在这里，我们使用具有不同小脑折叠量的近交系小鼠来研究这些预测。我们确定了一个关键时期，在那里折叠量在不同菌株之间存在差异。在这一时期，外部颗粒层(EGL)和下伏岩心之间差异膨胀水平的区域性变化与每个应变中的折叠量相关。此外，EGL的厚度在关键期间随波长的相应变化而进行区域调整。虽然表达SHH的浦肯野细胞的数量预测了折叠量，但不同菌株在EGL中的增殖率是相同的。然而，EGL内细胞分裂角度的区域性变化预测了EGL的切向扩张和厚度。细胞分裂角度可能是一种可调的机制，通过它可以对EGL的差异膨胀程度和厚度进行区域性调整，以设置折叠的数量和波长。
Modeling has proposed that the amount of neural tissue folding is set by the level of differential-expansion between tissue layers and that the wavelength is set by the thickness of the outer layer. Here we used inbred mouse strains with distinct amounts of cerebellar folding to investigate these predictions. We identified a critical period where the folding amount diverges between the strains. In this period, regional changes in the level of differential-expansion between the external granule layer (EGL) and underlying core correlate with the folding amount in each strain. Additionally, the thickness of the EGL is regionally adjusted during the critical period alongside corresponding changes in wavelength. While the number of SHH-expressing Purkinje cells predicts the folding amount, the proliferation rate in the EGL is the same between the strains. However, regional changes in the cell division angle within the EGL predicts both the tangential-expansion and thickness of the EGL. Cell division angle is likely a tunable mechanism whereby both the level of differential-expansion and thickness of the EGL are regionally tuned to set the amount and wavelength of folding.