Membrane interactions and self-association of components of the Ess/Type VII secretion system of Staphylococcus aureus.
Membrane interactions and self-association of components of the Ess/Type VII secretion system of Staphylococcus aureus.
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DOI：
10.1002/1873-3468.12065
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发表时间：
2016-02
期刊：
影响因子：
3.5
通讯作者：
Palmer T
中科院分区：
文献类型：
--
作者：
Jäger F;Zoltner M;Kneuper H;Hunter WN;Palmer T
Ess/III型蛋白分泌系统对致病性金黄色葡萄球菌的毒力是必不可少的，它依赖于四个核心膜蛋白Essa、EssB、ESSC和ESAA。在这里，我们使用交联和蓝色天然PAGE分析来表明EssB、ESSC和ESAA蛋白分别形成同源复合体。令人惊讶的是，这些成分似乎不能相互作用，也不能与Essa蛋白相互作用。我们进一步表明，在整个细胞中检测到的两个ESSC的高分子量多聚体不依赖于EsxA、EsxB或任何其他Ess组分的存在。
The Ess/Type VII protein secretion system, essential for virulence of pathogenic Staphylococcus aureus, is dependent upon the four core membrane proteins EssA, EssB, EssC and EsaA. Here, we use crosslinking and blue native PAGE analysis to show that the EssB, EssC and EsaA proteins individually form homomeric complexes. Surprisingly, these components appear unable to interact with each other, or with the EssA protein. We further show that two high molecular weight multimers of EssC detected in whole cells are not dependent upon the presence of EsxA, EsxB or any other Ess component for their assembly.