Noninvasive laser-induced photoacoustic tomography for structural and functional in vivo imaging of the brain
Noninvasive laser-induced photoacoustic tomography for structural and functional in vivo imaging of the brain
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DOI：
10.1038/nbt839
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发表时间：
2003-07-01
影响因子：
46.9
通讯作者：
Wang, LHV
中科院分区：
文献类型：
--
作者：
Wang, XD;Pang, YJ;Wang, LHV
基于光学对比度分析的成像技术可用于通过由与脑生理学和病理学相关的血容量、耗氧量和细胞肿胀的变化产生的光学信号来可视化神经系统的动态和功能特性。在这里，我们报告在体内无创性的经皮和经颅成像的结构和功能的大鼠大脑的激光诱导光声断层扫描（PAT）。PAT相对于纯光学成像的优势在于，它保留了固有的光学对比度特性，同时利用了超声的衍射限制的高空间分辨率。我们准确地绘制了大鼠大脑结构，有和没有病变，和功能性大脑血流动力学的变化，在周围的胡须桶皮质的皮质血管对胡须刺激。我们还成像高氧和缺氧引起的脑血流动力学变化。这种神经影像学模式有望在神经生理学，神经病理学和神经治疗中应用。
Imaging techniques based on optical contrast analysis can be used to visualize dynamic and functional properties of the nervous system via optical signals resulting from changes in blood volume, oxygen consumption and cellular swelling associated with brain physiology and pathology. Here we report in vivo noninvasive transdermal and transcranial imaging of the structure and function of rat brains by means of laser-induced photoacoustic tomography (PAT). The advantage of PAT over pure optical imaging is that it retains intrinsic optical contrast characteristics while taking advantage of the diffraction-limited high spatial resolution of ultrasound. We accurately mapped rat brain structures, with and without lesions, and functional cerebral hemodynamic changes in cortical blood vessels around the whisker-barrel cortex in response to whisker stimulation. We also imaged hyperoxia- and hypoxia-induced cerebral hemodynamic changes. This neuroimaging modality holds promise for applications in neurophysiology, neuropathology and neurotherapy.