Seven transmembrane domain receptor subtypes identified in NG108-15 cells by reverse transcription-polymerase chain reaction.
Seven transmembrane domain receptor subtypes identified in NG108-15 cells by reverse transcription-polymerase chain reaction.
复制标题
通过逆转录聚合酶链反应在 NG108-15 细胞中鉴定出七种跨膜结构域受体亚型。
DOI：
10.1006/bbrc.1994.2808
复制
发表时间：
1994
影响因子：
3.1
通讯作者：
Crabb,DW
中科院分区：
文献类型：
--
作者：
Chan,RJ;McBride,AW;Crabb,DW
NG108-15 neuroblastoma x glioma cells are widely used for the study of neurotransmitter receptors. We utilized reverse transcription-polymerase chain reaction to amplify members of the seven transmembrane domain class of G-protein linked receptors using RNA isolated from NG108-15 cells. Two complementary DNAs representing receptors were obtained; based upon comparison with the sequence database, they probably represent the murine dopamine D1Areceptor and a receptor closely related to the serotonin 5HT1Dreceptor subtype. The finding of the 5HT receptor subtype is of interest, as only the 5HT3subtype was previously identified in NG108-15 cells by pharmacological means. Certain responses of NG108-15 cells to serotonin have been described that do not appear to be mediated by known 5HT receptor subtypes. The cDNA we cloned may therefore represent an additional 5HT1Dsubclass.
