ASSAY OF PROTEINS IN PRESENCE OF INTERFERING MATERIALS
ASSAY OF PROTEINS IN PRESENCE OF INTERFERING MATERIALS
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DOI：
10.1016/s0003-2697(76)80064-4
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发表时间：
1976-01-01
影响因子：
2.9
通讯作者：
WEINSTEIN, D
中科院分区：
文献类型：
--
作者：
BENSADOUN, A;WEINSTEIN, D
Lowry蛋白测定法是一种敏感但高度非特异性的方法。对标准Lowry蛋白质测定法进行了修改，以便在存在干扰化学品的情况下测定蛋白质。该方法基于这样的观察，即在125 μ g/ml脱氧胆酸钠存在下，牛血清白蛋白（5-50 μ g）被6%三氯乙酸可重复地沉淀（90-104%）。蔗糖、甘油、Tris-HCl、Tricine和EDTA的干扰可消除。也可测定含有载体两性电解质的蛋白质样品，条件是将其NaCl浓度调节至1 M。
The Lowry protein assay is a sensitive but highly nonspecific procedure. The standard Lowry protein assay was modified, so that protein can be assayed in the presence of interfering chemicals. The method is based on the observation that in the presence of 125 .mu.g/ml of Na-deoxycholate, bovine serum albumin (5-50 .mu.g) is reproducibly precipitated (90-104%) by 6% trichloroacetic acid. Interference by sucrose, glycerol, Tris-HCl, Tricine and EDTA can be eliminated. Protein samples containing carrier ampholytes can also be assayed provided their NaCl concentration is adjusted to 1 M.