K-ras codon 12 mutation determines the polypoid growth of colorectral cancer.
K-ras codon 12 mutation determines the polypoid growth of colorectral cancer.
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K-ras密码子12突变决定了结直肠癌的息肉样生长。
作者：
J. Chiang;Y. Chou;T. Chou
人们普遍认为结直肠癌的发生遵循腺瘤-腺癌的顺序。然而，结直肠癌（CRC）有两种形态不同的亚型，息肉样癌和溃疡性癌。我们进行了一项比较研究，以阐明在息肉样型和溃疡型CRC（两种形态不同的CRC类型）之间是否存在一些常见的遗传改变（包括K-ras、p53、DCC、APC和Rb基因的突变）的不同组合。通过基于pcr的RFLP、单链构象多态性和杂合缺失分析，我们发现K-ras密码子12突变优先参与息肉样瘤（P < 0.0001）。5q、17p、18q染色体和Rb基因的p53点突变或杂合性缺失与两种形态类型CRC的进展均无显著相关。因此，分子遗传改变的不同组合可能参与形态不同类型的结直肠癌发生，K-ras密码子12突变可能在结直肠癌的息肉样生长中起重要作用。这些结果揭示了K-ras癌基因在结直肠癌发生中的功能，并可能对未来设计结直肠癌患者的遗传筛查方案、确定预后和治疗具有重要意义。
Colorectal carcinogenesis is widely thought to follow the adenoma-adenocarcinoma sequence. However, there are two morphologically distinct subtypes of colorectal cancer (CRC), polypoid and ulcerative. We conducted a comparative study to clarify whether different combinations of some commonly involved genetic alterations (including mutations in K-ras, p53, DCC, APC, and Rb genes) may exist between polypoid- and ulcerative-type CRCs, the two morphologically distinct types of CRC. By using PCR-based RFLP, single-strand conformational polymorphism, and loss of heterozygosity analysis, we found that K-ras codon 12 mutation was preferentially involved in polypoid tumor (P < 0.0001). There were no other significant correlations with p53 point mutation or loss of heterozygosity in chromosomes 5q, 17p, and 18q and Rb gene, which have been suggested to be involved in the progression of CRC of both morphological types. Therefore, different combinations of molecular genetic alterations may be involved in morphologically distinct types of colorectal carcinogenesis, and the K-ras codon 12 mutations may play an important role in polypoid growth of CRC. These results shed light on the function of K-ras oncogenes involved in colorectal carcinogenesis and may be important in the future design of genetic screening programs, determination of prognosis, and treatment for patients with CRC.
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影响因子：
8
作者：
S. Kahn;Wei Jiang;Culbertson Ta;Weinstein Ib;Williams Gm;N. Tomita;Z. Ronai
通讯作者：
S. Kahn;Wei Jiang;Culbertson Ta;Weinstein Ib;Williams Gm;N. Tomita;Z. Ronai
K-ras 突变是叙利亚金仓鼠胰管癌发生的早期事件。
影响因子：
11.2
作者：
Cerny,WL;Mangold,KA;Scarpelli,DG
通讯作者：
Scarpelli,DG
小鼠皮肤癌发生过程中血管内皮生长因子/血管通透性因子的上调与恶性进展状态和激活的 H-ras 表达水平相关。
DOI：
--
发表时间：
1996
期刊：
Cancer research.
影响因子：
--
作者：
Larcher,F;Robles,AI;Duran,H;Murillas,R;Quintanilla,M;Cano,A;Conti,CJ;Jorcano,JL
通讯作者：
Jorcano,JL
影响因子：
29.4
作者：
KERN, SE;REDSTON, M;KINZLER, KW
通讯作者：
KINZLER, KW
D17S513 基因座的二核苷酸重复多态性。
影响因子：
14.9
作者：
Oliphant,AR;Wright,EC;Swensen,J;Gruis,NA;Goldgar,D;Skolnick,MH
通讯作者：
Skolnick,MH