Neutral lipids and the assessment of infectivity in entomopathogenic nematodes: Observations on four Steinernema species
Neutral lipids and the assessment of infectivity in entomopathogenic nematodes: Observations on four Steinernema species
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DOI：
10.1017/s0031182096008748
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发表时间：
1997-05-01
期刊：
影响因子：
2.4
通讯作者：
Wright, DJ
中科院分区：
文献类型：
--
作者：
Patel, MN;Stolinski, M;Wright, DJ
本文提出了一个油红O染色法测定果荚斯氏线虫、斯氏线虫和线虫感染性幼虫中性脂质的8点视觉指数。riobravis（Biosys 355）、S. feltiae（UK76）和S. glaseri（NC）.视觉指数被认为是一个可靠的和快速的方法，用于确定相对中性脂质含量的个人IJS，并通过气相色谱定量验证。中性脂质的利用和感染性IJs存储在蒸馏水在25 ℃之间的关系也进行了研究，并在昆虫病原线虫的中性脂质利用的第一个定量结果报告。新鲜收获的S. carpocapsae，S. riobravis，S. feltiae和S. glaseri分别为31、31、24和26%干重。斯氏线虫对中性脂质的利用呈S型，而S. Riobravis以几乎恒定的速率使用中性脂质。这两个物种的生存期为120至135天，而S。feltiae和S. Glaseri存活> 450天，并且在260天的储存期内具有较慢的脂质利用率。油红O染色结果显示，尽管初始脂质指数相同，但各群体中个体IJs对脂质的利用率不同。结果表明，S. riobravis，S. feltiae和S. glaseri随着脂质利用而下降。相反，S.即使在相对较低的脂质水平下，carpocapsae也保持高水平的感染性。因此，中性脂质含量被认为是一个合适的指标，感染性的S。riobravis，S. feltiae和S. glaseri，而S.果荚类
An 8-point visual index was developed for Oil Red O staining of neutral lipids in infective juveniles (IJs) of Steinernema carpocapsae (All), S. riobravis (Biosys 355), S. feltiae (UK76) and S. glaseri (NC). The visual index was found to be a reliable and rapid method for determining the relative neutral lipid content of individual IJs and was validated quantitatively by gas chromatography. The relationship between neutral lipid utilization and infectivity of IJs stored in distilled water at 25 degrees C was also investigated and the first quantitative results on neutral lipid utilization in entomopathogenic nematodes are reported. Neutral lipid contents of freshly harvested IJs of S. carpocapsae, S. riobravis, S. feltiae and S. glaseri were 31, 31, 24 and 26 % dry wt, respectively. Steinernema carpocapsae showed a sigmoidal pattern for neutral lipid utilization while S. riobravis used neutral lipids at an almost constant rate. Survivorship of these two species ranged between 120 and 135 days, whereas S. feltiae and S. glaseri lived > 450 days and had a slower rate of lipid utilization during a 260 day storage period. Oil Red O staining showed that individual IJs in each population utilized lipids at different rates, even though they had the same initial lipid index. The infectivity of S. riobravis, S. feltiae and S. glaseri declined with lipid utilization. In contrast, S. carpocapsae maintained a high level of infectivity even at relatively low lipid levels. Therefore, neutral lipid content was found to be a suitable indicator of infectivity for S. riobravis, S. feltiae and S. glaseri but not for S. carpocapsae.