Opiate binding to subcellular fractions from guinea pig ileum.
Opiate binding to subcellular fractions from guinea pig ileum.
复制标题
阿片与豚鼠回肠亚细胞部分的结合。
DOI：
10.1016/0024-3205(84)90622-2
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发表时间：
1984
期刊：
影响因子：
6.1
通讯作者：
Cooper,JR
中科院分区：
文献类型：
--
作者：
Glasel,JA;Bradbury,WM;Venn,RF;Reese,JH;Cooper,JR
本文研究了~H-埃托啡和~H-D-丙氨酸-D-亮氨酸-5-脑啡肽与豚鼠回肠匀浆突触体组分和微体组分阿片受体的结合。结果表明，埃托啡各组分的离解常数相同。纯化后的突触体组分与依托吗啡的结合量比其他组分大5倍。脑啡肽衍生物与微粒体组分的结合解离常数大于依托吗啡，结合量比其他组分低3倍。这些结果与中枢神经系统亚细胞组分结合的情况相反。
Binding of3H-etorphine and3H-D-Ala2-D-Leu5-enkephalin to opiate receptors in synaptosomal and microsomal fractions prepared from guinea pig ileum homogenates has been studied. It is found that the dissociation constants for etorphine from all fractions are the same. The binding capacity for etorphine for the purified synaptosomal fraction is greater than for other fractions by a factor of 5. For the enkephalin derivative binding to the microsomal fraction the dissociation constant is greater than for etorphine while the binding capacity is a factor of 3 lower. These results are in contrast to the case for binding to central nervous system subcellular fractions.