Circular RNA circIGF2BP3 Promotes the Proliferation and Differentiation of Chicken Primary Myoblasts.
Circular RNA circIGF2BP3 Promotes the Proliferation and Differentiation of Chicken Primary Myoblasts.
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DOI：
10.3390/ijms242115545
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发表时间：
2023-10-24
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
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动物肉的质量和数量与骨骼肌的发育密切相关，而骨骼肌的发育又由成肌细胞和骨骼肌卫星细胞（SMSCs）决定。环状RNA是通过mRNA前体的特异性反向剪接形成的内源性RNA衍生物，具有通过与miRNA结合或在转录水平上调节参与肌肉生长的基因表达来影响肌肉发育的潜力。先前对鸡肝脏组织中circRNA的高通量测序揭示了一种环状转录物circIGF 2BP 3，其来源于编码胰岛素样生长因子2 mRNA结合蛋白3（IGF 2BP 3）的基因。在这项研究中，我们通过RNase R酶耐受性测定证实了circIGF 2BP 3天然环状分子的存在。RT-qPCR结果显示，在14、18日龄和7周龄时，CircIGF 2BP 3基因在粤西曲羽鸡胸肌和大腿肌肉中有较高的表达。值得注意的是，它的表达在胚胎发育期间增加，随后在出生后迅速减少。以及使用RT-qPCR、Edu、CCK-8、免疫荧光和Western印迹技术，我们证明过表达circIGF 2BP 3可以通过上调增殖细胞核抗原（PCNA）、细胞周期蛋白D1（CCND 1）、细胞周期蛋白E1（CCNE 1）、细胞周期蛋白依赖性激酶2（CDK 2）、肌球蛋白重链（MyHC）、成肌细胞决定蛋白1（MyoD 1）、肌细胞生成素（MyoG）和Myomaker。综上所述，circIGF 2BP 3促进鸡成肌细胞的增殖和分化。本研究为进一步研究circIGF 2BP 3在成肌细胞增殖和分化中的生物学功能和机制奠定了基础。
The quality and quantity of animal meat are closely related to the development of skeletal muscle, which, in turn, is determined by myogenic cells, including myoblasts and skeletal muscle satellite cells (SMSCs). Circular RNA, an endogenous RNA derivative formed through specific reverse splicing in mRNA precursors, has the potential to influence muscle development by binding to miRNAs or regulating gene expression involved in muscular growth at the transcriptional level. Previous high-throughput sequencing of circRNA in chicken liver tissue revealed a circular transcript, circIGF2BP3, derived from the gene encoding insulin-like growth factor 2 mRNA binding protein 3 (IGF2BP3). In this study, we confirmed the presence of the natural circular molecule of circIGF2BP3 through an RNase R enzyme tolerance assay. RT-qPCR results showed high circIGF2BP3 expression in the pectoral and thigh muscles of Yuexi frizzled feather chickens at embryonic ages 14 and 18, as well as at 7 weeks post-hatch. Notably, its expression increased during embryonic development, followed by a rapid decrease after birth. As well as using RT-qPCR, Edu, CCK-8, immunofluorescence, and Western blot techniques, we demonstrated that overexpressing circIGF2BP3 could promote the proliferation and differentiation of chicken primary myoblasts through upregulating genes such as proliferating cell nuclear antigen (PCNA), cyclin D1 (CCND1), cyclin E1 (CCNE1), cyclin dependent kinase 2 (CDK2), myosin heavy chain (MyHC), myoblast-determining 1 (MyoD1), myogenin (MyoG), and Myomaker. In conclusion, circIGF2BP3 promotes the proliferation and differentiation of myoblasts in chickens. This study establishes a foundation for further investigation into the biological functions and mechanisms of circIGF2BP3 in myoblasts proliferation and differentiation.
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影响因子：
37.3
作者：
Liu Z;Wang T;She Y;Wu K;Gu S;Li L;Dong C;Chen C;Zhou Y
通讯作者：
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影响因子：
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影响因子：
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作者：
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