Acclimation of Emiliania huxleyi (1516) to nutrient limitation involves precise modification of the proteome to scavenge alternative sources of N and P.
Acclimation of Emiliania huxleyi (1516) to nutrient limitation involves precise modification of the proteome to scavenge alternative sources of N and P.
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DOI：
10.1111/1462-2920.12957
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发表时间：
2015-10
影响因子：
5.1
通讯作者：
Geider RJ
中科院分区：
文献类型：
--
作者：
McKew BA;Metodieva G;Raines CA;Metodiev MV;Geider RJ
氮或磷的有效性限制海洋初级生产是常见的。E miliania huxleyi是一种普遍存在的植物寄生虫，在初级生产，碳酸钙沉淀和二甲基硫的生产中起着关键作用，通常在夏季开始时在无机营养盐浓度较低的中纬度地区开花。为了了解允许这种开花的生理机制，我们研究了E . huxleyi（菌株1516）的蛋白质组如何响应N和P限制。我们观察到适度的变化，尽管大的生理变化（如细胞生物量，C，N和P）与生长速率的营养限制的蛋白质组。然而，驯化营养限制涉及大量的转运铵和硝酸盐的氮限制和磷限制下磷酸盐的显着增加。更值得注意的是，参与获得有机形式的N和P的蛋白质大幅增加，包括尿素和氨基酸/多胺转运蛋白以及N限制下的许多C-N水解酶，以及P限制下碱性磷酸酶的大幅上调。这种高度针对性的蛋白质组重组，以清除有机形式的大量营养素，提供了独特的见解的分子机制，支持如何E .赫胥黎找到了它的生态位，在表面沃茨开花耗尽无机营养素。
Limitation of marine primary production by the availability of nitrogen or phosphorus is common. E miliania huxleyi, a ubiquitous phytoplankter that plays key roles in primary production, calcium carbonate precipitation and production of dimethyl sulfide, often blooms in mid‐latitude at the beginning of summer when inorganic nutrient concentrations are low. To understand physiological mechanisms that allow such blooms, we examined how the proteome of E . huxleyi (strain 1516) responds to N and P limitation. We observed modest changes in much of the proteome despite large physiological changes (e.g. cellular biomass, C, N and P) associated with nutrient limitation of growth rate. Acclimation to nutrient limitation did however involve significant increases in the abundance of transporters for ammonium and nitrate under N limitation and for phosphate under P limitation. More notable were large increases in proteins involved in the acquisition of organic forms of N and P, including urea and amino acid/polyamine transporters and numerous C‐N hydrolases under N limitation and a large upregulation of alkaline phosphatase under P limitation. This highly targeted reorganization of the proteome towards scavenging organic forms of macronutrients gives unique insight into the molecular mechanisms that underpin how E . huxleyi has found its niche to bloom in surface waters depleted of inorganic nutrients.