Quantitative comparison of cancer and normal cell adhesion using organosilane monolayer templates: An experimental study on the anti-adhesion effect of green-tea catechins
Quantitative comparison of cancer and normal cell adhesion using organosilane monolayer templates: An experimental study on the anti-adhesion effect of green-tea catechins
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使用有机硅烷单层模板定量比较癌症和正常细胞粘附：绿茶儿茶素抗粘附作用的实验研究
DOI：
10.1007/s11626-016-0049-6
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发表时间：
2016
期刊：
影响因子：
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通讯作者：
Takashi Tanii
中科院分区：
文献类型：
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作者：
Rumi Sakamoto;Eisuke Kakinuma;Kentaro Masuda;Yuko Takeuchi;Kosaku Ito;Kentaro Iketaki;Takahisa Matsuzaki;Seiichiro Nakabayashi;Hiroshi Y. Yoshikawa;Hideaki Yamamoto;Yuko Sato;Takashi Tanii
绿色茶的主要成分，（−）-表没食子儿茶素-3-O-没食子酸酯（EGCG），已知具有癌症特异性化学预防作用。在目前的工作中，我们研究如何抑制细胞粘附的EGCG通过比较粘附的人胰腺癌细胞（AsPC-1和BxPC-3）和他们的对手，正常的人胚胎胰腺衍生细胞（1C 3D 3），在儿茶素含介质中使用有机硅烷单层模板（OMT）。本研究的目的是（1）评价OMT测定细胞粘附的定量性，（2）显示绿茶儿茶素如何以癌症特异性方式抑制细胞粘附。对于第一个目的，使用OMT比较癌细胞和正常细胞的粘附。还评估了不同类型的儿茶素如EGCG、（-）-表儿茶素-3-O-没食子酸酯（ECG）和（-）-表儿茶素（EC）中的细胞粘附。结果表明，绿茶儿茶素的抗粘附作用具有肿瘤特异性，其作用大小顺序为EGCG> ECG>EC。计算结果与文献报道的数据吻合较好，表明了新方法的定量性。对于第二个目的，OMT上的细胞的接触面积通过反射干涉对比显微镜测量。癌细胞的细胞-OMT接触面积随着EGCG浓度的增加而减小，而正常细胞的细胞-OMT接触面积保持恒定。结果揭示了EGCG对癌细胞粘附的双重作用-抑制细胞粘附到候选粘附位点并减少细胞的接触面积-并验证了OMT作为筛选癌细胞粘附的工具的用途。
The main constituent of green tea, (−)-Epigallocatechin-3-O-gallate (EGCG), is known to have cancer-specific chemopreventive effects. In the present work, we investigated how EGCG suppresses cell adhesion by comparing the adhesion of human pancreatic cancer cells (AsPC-1 and BxPC-3) and their counterpart, normal human embryonic pancreas-derived cells (1C3D3), in catechin-containing media using organosilane monolayer templates (OMTs). The purpose of this work is (1) to evaluate the quantitativeness in the measurement of cell adhesion with the OMT and (2) to show how green-tea catechins suppress cell adhesion in a cancer-specific manner. For the first purpose, the adhesion of cancer and normal cells was compared using the OMT. The cell adhesion in different type of catechins such as EGCG, (−)-Epicatechin-3-O-gallate (ECG) and (−)-Epicatechin (EC) was also evaluated. The measurements revealed that the anti-adhesion effect of green-tea catechins is cancer-specific, and the order is EGCG≫ECG>EC. The results agree well with the data reported to date, showing the quantitativeness of the new method. For the second purpose, the contact area of cells on the OMT was measured by reflection interference contrast microscopy. The cell-OMT contact area of cancer cells decreases with increasing EGCG concentration, whereas that of normal cells remains constant. The results reveal a twofold action of EGCG on cancer cell adhesion—suppressing cell attachment to a candidate adhesion site and decreasing the contact area of the cells—and validates the use of OMT as a tool for screening cancer cell adhesion.