Increased Calcineurin A Expression Is Associated with a Lower Relapse-Free Survival Rate after Colorectal Cancer Surgery.
Increased Calcineurin A Expression Is Associated with a Lower Relapse-Free Survival Rate after Colorectal Cancer Surgery.
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钙调神经磷酸酶 A 表达增加与结直肠癌手术后较低的无复发生存率相关。
作者：
Niitsu H;Hinoi T;Sentani K;Mukai S;Adachi T;Saito Y;Miguchi M;Kochi M; Sada H;Oue N;Yasui W;Ohdan H.
钙调神经磷酸酶在结直肠癌（CRC）中的表达增加已被报道。虽然已经提出了致癌功能，但其临床意义仍不清楚。我们在此研究钙调神经磷酸酶的表达作为一个预后的生物标志物，在接受根治性手术的患者为I-III期CRC.MethodsIn 121例I-III期CRC治疗在广岛大学之间的1997年和2003年，钙调神经磷酸酶A的表达进行了检查，使用免疫组化（IHC）染色的手术标本。如果在癌变区域内观察到任何IHC染色的细胞，则认为标本为钙调神经磷酸酶A阳性，并比较IHC阳性和阴性组之间的临床病理特征和生存结局。（范围：0-80%;四分位数间距：0-22.5%）在癌性区域内染色。在121例病例中，81例被确定为IHC阳性，40例被确定为阴性。多因素分析显示，钙调神经磷酸酶A阳性表达、UICC-TNM分期与低无复发生存率相关（hazard ratio= 2.92，95%CI：1.27-7.92，p= 0.010）。
ObjectiveIncreased expression of calcineurin in colorectal cancer (CRC) has been reported. Although the oncogenic function has been suggested, the clinical relevance is still unclear. We herein studied calcineurin expression as a prognostic biomarker in patients receiving curative surgery for stages I-III CRC.MethodsIn 121 patients with stages I-III CRC treated at Hiroshima University between 1997 and 2003, calcineurin A expression was examined using immunohistochemistry (IHC) staining of surgical specimens. Specimens were considered positive for calcineurin A if any IHC-stained cells were observed within the carcinomatous area, and clinicopathological characteristics and survival outcomes were compared between IHC-positive and-negative groups.ResultsCalcineurin A was preferentially expressed in the cytoplasm of cancer cells, and a median of 8% of the cells (range: 0-80%; interquartile range: 0-22.5%) were stained within the carcinomatous areas. Of 121 cases, 81 were determined as IHC positive while 40 were determined to be negative. Positive expression of calcineurin A, as well UICC-TNM stage, was associated with low relapse-free survival (RFS) rates in multivariate analyses (hazard ratio= 2.92; 95% CI: 1.27-7.92; p= 0.010).ConclusionIncreased calcineurin A expression is associated with lower RFS rates and may have clinical value in predicting recurrence.