Differential expression of miRNAs in the nervous system of a rat model of bilateral sciatic nerve chronic constriction injury
Differential expression of miRNAs in the nervous system of a rat model of bilateral sciatic nerve chronic constriction injury
复制标题
双侧坐骨神经慢性缩窄性损伤模型神经系统中miRNA的差异表达
DOI：
10.3892/ijmm.2013.1381
复制
发表时间：
2013-07-01
影响因子：
5.4
通讯作者：
Huang, Yuguang
中科院分区：
文献类型：
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作者：
Li, Haixia;Shen, Le;Huang, Yuguang
慢性神经病理性疼痛与伤害性通路不同区域基因表达的整体变化相关。MicroRNA（miRNAs）是一类长度约为22 nt的非编码小RNA，能够在转录后调节数百种不同的基因。本研究的目的是使用双侧坐骨神经慢性压迫性损伤（bCCI）诱导的人类神经病理性疼痛大鼠模型来确定疼痛传递通路不同区域中的miRNA表达模式。使用微阵列分析和定量逆转录酶-PCR，我们观察到一个显着上调miR-341的表达在背根神经节（DRG），但不是在脊髓背角（SDH），海马或前扣带皮层（ACC），在神经病理性疼痛的大鼠相比，在幼稚和假手术组的大鼠。相比之下，miR-203、miR-181 a-1* 和miR-541* 在神经病理性疼痛大鼠的SDH中的表达显著降低。我们的数据表明，在神经性疼痛条件下，miR-341在DRG中上调，而miR-203、miR-181 a-1* 和miR-541* 在SDH中下调。因此，miRNAs在神经系统中的差异表达可能在慢性疼痛的发展中起作用。这些观察结果可能有助于开发神经性疼痛的新治疗方法，这可能涉及局部区域的miRNA基因治疗。
Chronic neuropathic pain is associated with global changes in gene expression in different areas of the nociceptive pathway. MicroRNAs (miRNAs) are small (similar to 22 nt long) non-coding RNAs, which are able to regulate hundreds of different genes post-transcriptionally. The aim of this study was to determine the miRNA expression patterns in the different regions of the pain transmission pathway using a rat model of human neuropathic pain induced by bilateral sciatic nerve chronic constriction injury (bCCI). Using microarray analysis and quantitative reverse transcriptase-PCR, we observed a significant upregulation in miR-341 expression in the dorsal root ganglion (DRG), but not in the spinal dorsal horn (SDH), hippocampus or anterior cingulate cortex (ACC), in the rats with neuropathic pain compared to rats in the naive and sham-operated groups. By contrast, the expression of miR-203, miR-181a-1* and miR-541* was significantly reduced in the SDH of rats with neuropathic pain. Our data indicate that miR-341 is upregulated in the DRG, whereas miR-203, miR-181a-1* and miR-541* are downregulated in the SDH under neuropathic pain conditions. Thus, the differential expression of miRNAs in the nervous system may play a role in the development of chronic pain. These observations may aid in the development of novel treatment methods for neuropathic pain, which may involve miRNA gene therapy in local regions.