When a module is not a domain: the case of the REJ module and the redefinition of the architecture of polycystin-1.
When a module is not a domain: the case of the REJ module and the redefinition of the architecture of polycystin-1.
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当模块不是域时：REJ模块的情况和多囊蛋白-1架构的重新定义。
DOI：
10.1042/bj20101810
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Pfuhl,Mark
中科院分区：
文献类型：
--
作者：
Schröder,Samantha;Fraternali,Franca;Quan,Xueping;Scott,David;Qian,Feng;Pfuhl,Mark
一组被称为多囊肾病 1 家族的细胞表面受体的细胞外区域，其中含有多囊蛋白-1，但有争议的是，它被描述为在多肽的同一部分中含有四个 FNIII（纤连蛋白 III 型）结构域或一个 REJ（卵果冻蛋白受体）模块。受最近原子力显微镜工作的刺激，我们用显示进化关系的 FNIII 序列图谱重新检查了这四个结构域的相似性。其中两个预测的结构域可以在细菌中表达并重新折叠以产生适合生物物理研究的蛋白质，其中一个可溶性表达。 CD 光谱显示这两个结构域都含有大量的 β-折叠，与理论预测非常一致。从高度协作的热和尿素展开曲线中获得了独立折叠作为域的确认。一维核磁共振谱高场区域中峰的出色分散证实了疏水核的存在。分析超速离心和分析凝胶过滤与 NMR 谱中的窄线宽非常吻合，即至少一个域是单体。基于理论和实验相结合的分析，我们表明多囊蛋白-1的胞外部分确实包含β-折叠结构域，可能是FNIII，因此，REJ模块不是单个结构域。
The extracellular region of a group of cell-surface receptors known as the polycystic kidney disease 1 family, containing, among others, polycystin-1, has been controversially described as containing four FNIII (fibronectin type III) domains or one REJ (receptor of egg jelly protein) module in the same portion of polypeptide. Stimulated by recent atomic force microscopy work, we re-examined the similarity of these four domains with a FNIII sequence profile showing the evolutionary relationship. Two of the predicted domains could be expressed in bacteria and refolded to give a protein suitable for biophysical study, and one of these expressed solubly. CD spectroscopy showed that both domains contain a significant amount of β-sheet, in good agreement with theoretical predictions. Confirmation of independent folding as a domain is obtained from highly co-operative thermal and urea unfolding curves. Excellent dispersion of peaks in the high-field region of one-dimensional NMR spectra confirms the presence of a hydrophobic core. Analytical ultracentrifugation and analytical gel filtration agree very well with the narrow linewidths in the NMR spectra that at least one of the domains is monomeric. On the basis of this combined theoretical and experimental analysis, we show that the extracellular portion of polycystin-1 does indeed contain β-sheet domains, probably FNIII, and that, consequently, the REJ module is not a single domain.
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影响因子：
2.9
作者：
C. Penkett;C. Dobson;L. Smith;J. R. Bright;A. Pickford;I. Campbell;J. Potts
通讯作者：
J. Potts
影响因子：
64.5
作者：
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真正的多囊蛋白-1 的体外和体内生化表征。
DOI：
10.1053/ajkd.2001.29282
发表时间：
2001
期刊：
American journal of kidney diseases : the official journal of the National Kidney Foundation.
影响因子：
--
作者：
Boletta,A;Qian,F;Onuchic,LF;Bragonzi,A;Cortese,M;Deen,PM;Courtoy,PJ;Soria,MR;Devuyst,O;Monaco,L;Germino,GG
通讯作者：
Germino,GG
影响因子：
158.5
作者：
Grantham, Jared J.
通讯作者：
Grantham, Jared J.
影响因子：
5.6
作者：
Pfuhl, M;Improta, S;Pastore, A
通讯作者：
Pastore, A