MAGNITUDE, KINETICS, AND DURATION OF VIBRIOCIDAL ANTIBODY-RESPONSES IN NORTH AMERICANS AFTER INGESTION OF VIBRIO-CHOLERAE
MAGNITUDE, KINETICS, AND DURATION OF VIBRIOCIDAL ANTIBODY-RESPONSES IN NORTH AMERICANS AFTER INGESTION OF VIBRIO-CHOLERAE
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DOI：
10.1093/infdis/145.4.465
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发表时间：
1982-01-01
影响因子：
6.4
通讯作者：
CRAIG, JP
中科院分区：
文献类型：
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作者：
CLEMENTS, ML;LEVINE, MM;CRAIG, JP
用试管稀释法检测了147名志愿者摄入103-106株霍乱弧菌前后血清中的杀弧菌抗体。在110名排出霍乱弧菌的人中，97%的人在挑战后滴度显著增加。在12%的细菌学确认的感染中，仅同源（稻叶或小川）血清型的滴度显著增加。杀弧菌抗体的水平在1-6个月之间显著降低。但通常仍高于基线值。用IgG酶联免疫吸附试验（ELISA）、家兔皮肤通透性试验和肾上腺细胞（小鼠肾上腺皮质腺瘤Y-1细胞）技术检测了73对攻毒前后血清的抗霍乱弧菌毒素水平。在血清学检测临床和亚临床感染中，杀弧菌试验的结果与最敏感的抗毒素试验ELISA的结果最接近。最近的感染可以准确地血清学诊断水平的杀弧菌抗体和抗毒素（IgG ELISA）在恢复期血清。
Sera from 147 volunteers were examined for vibriocidal antibody by the tube-dilution method before and after they ingested 103-106 V. cholerae organisms. Titers increased significantly after challenge in 97% of 110 persons who excreted V. cholerae. In 12% of the bacteriologically confirmed infections, the titer increased significantly only to the homologous (Inaba or Ogawa) serotype. Levels of vibriocidal antibody decreased substantially between 1-6 mo. after challenge, but they usually remained elevated over base-line values. Levels of antitoxin to V. cholerae were measured by IgG enzyme-linked immunosorbent assay (ELISA), rabbit skin permeability tests and adrenal cell (mouse adrenocortical adenoma Y-1 cell) techniques in 73 pairs of prechallenge and postchallenge sera. Results of the vibriocidal assay agreed most closely with those of the ELISA, the most sensitive antitoxin assay, in serologically detecting clinical and subclinical infections. Recent infection could be accurately serodiagnosed by levels of vibriocidal antibody and antitoxin (by IgG ELISA) in a convalescent-phase serum.