Construction of a practical SCAR marker linked to clubroot resistance in Chinese cabbage, with intensive analysis of HC352b genes
Construction of a practical SCAR marker linked to clubroot resistance in Chinese cabbage, with intensive analysis of HC352b genes
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DOI：
10.2503/jjshs1.77.150
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发表时间：
2008-04-01
影响因子：
--
通讯作者：
Matsumoto, Etsuo
中科院分区：
文献类型：
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作者：
Hayashida, Nobuaki;Takabatake, Yuri;Matsumoto, Etsuo
通过对大白菜根肿病抗性基因CRa位点的分析，发现一个与CRa位点紧密连锁的限制性片段长度多态性标记HC 352 b。北京亚种（ssp pekinensis）。不幸的是，RFLP模式，包括由cDNA克隆HC 352检测到的HC 352 b，在其解释中是复杂和混乱的，因为它代表多拷贝位点。为了给CR育种提供一个实用的HC 352 b标记，本研究沿着对HC 352 b基因的分析，构建了一个序列特征扩增区（SCAR）标记，并对该标记进行了CR育种评价。通过鉴定HC 352同源基因及其限制性位点，采用CRa阳性和阴性双单倍体（DH）系来实现信号的示意性表征。分析来自一组HC 352同源基因的基因组序列信息以鉴定CRa连锁的paraffin HC 352 b，随后成功指定SCAR标记。这正确地预测了F-2和回交后代的所有测试个体的CR表型。
A restriction fragment length polymorphism (RFLP) marker, HC352b, has identified as closely linked to the CRa locus responsible for clubroot resistance (CR) in a study dissecting CR loci in Chinese cabbage (Brassica rapa L. ssp pekinensis). Unfortunately, the RFLP pattern, including HC352b detected by the cDNA clone HC352, was complicated and confused in its interpretation because it represented multiple-copy loci. To provide a practical HC352b marker for CR breeding programs, a sequence characterized amplified region (SCAR) marker was constructed along the analysis of HC352 genes, followed by its evaluation for CR selection. Schematic characterization of signals was achieved by identification of HC352 homologous genes with their restriction sites, employing CRa-positive and -negative doubled haploid (DH) lines. Genomic sequence information from a set of HC352 homologous genes was analyzed to identify the CRa linked paralog HC352b, followed by the successful designation of a SCAR marker. This correctly predicted the CR phenotypes of all tested individuals of F-2 and back-crossed progenies.