Neuropeptides promote neutrophil adherence to endothelial cell monolayers.
Neuropeptides promote neutrophil adherence to endothelial cell monolayers.
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神经肽促进中性粒细胞粘附至内皮细胞单层。
DOI：
10.1152/ajpgi.1992.263.5.g678
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Granger,DN
中科院分区：
文献类型：
--
作者：
Zimmerman,BJ;Anderson,DC;Granger,DN
本研究的目的是确定是否P物质和降钙素基因相关肽（CGRP），在生理相关浓度，影响白细胞内皮细胞粘附。在存在或不存在P物质或CGRP（10（-11）M）的情况下，将人脐静脉内皮细胞（HUVEC）的汇合单层与新鲜分离的人嗜中性粒细胞孵育（40分钟）。P物质和CGRP均引起中性粒细胞粘附HUVEC的显著增加（2倍）。针对白细胞粘附糖蛋白CD 11/CD 18（MAb IB 4）和L-选择素（MAb DREG 56）的单克隆抗体（MAb）不减弱P物质诱导的粘附。针对内皮细胞粘附分子E-选择素（MAb CL 2）和ICAM-1（MAb R6.5）的抗体对P物质诱导的中性粒细胞粘附也没有影响。当MAb IB 4、DREG 56、CL 2或R6.5与CGRP刺激的中性粒细胞和内皮细胞共孵育时，获得了类似的结果。佛波醇12-肉豆蔻酸酯13-乙酸酯刺激的中性粒细胞粘附显着减弱单克隆抗体IB 4，表明CD 11/CD 18参与了这一粘附过程。本研究的结果表明：1）神经肽P物质和CGRP促进中性粒细胞粘附到微静脉内皮; 2）神经肽诱导的粘附不是由粘附分子CD 11/CD 18、L-选择素、E-选择素或ICAM-1介导的。
The objective of this study was to determine whether substance P and calcitonin gene-related peptide (CGRP), at physiologically relevant concentrations, affect leukocyte-endothelial cell adhesion. Confluent monolayers of human umbilical vein endothelial cells (HUVEC) were incubated (40 min) with freshly isolated human neutrophils in the presence or absence of substance P or CGRP (10(-11) M). Both substance P and CGRP caused a significant increase (2-fold) in neutrophil adherence to HUVEC. Monoclonal antibodies (MAb) directed against the leukocyte adhesion glycoproteins CD11/CD18 (MAb IB4) and L-selectin (MAb DREG56) did not attenuate substance P-induced adhesion. Antibodies directed against the endothelial cell adhesion molecules E-selectin (MAb CL2) and ICAM-1 (MAb R6.5) were also without effect on substance P-induced neutrophil adhesion. Similar results were obtained when either MAb IB4, DREG56, CL2, or R6.5 was coincubated with CGRP-stimulated neutrophils and endothelial cells. Phorbol 12-myristate 13-acetate-stimulated neutrophil adherence was significantly attenuated by MAb IB4, indicating that CD11/CD18 participates in this adhesion process. The results of this study indicate that 1) the neuropeptides substance P and CGRP promote neutrophil adherence to venular endothelium and 2) the neuropeptide-induced adhesion is not mediated by the adhesion molecules CD11/CD18, L-selectin, E-selectin, or ICAM-1.