PORCINE RESPIRATORY CORONAVIRUS DIFFERS FROM TRANSMISSIBLE GASTROENTERITIS VIRUS BY A FEW GENOMIC DELETIONS
PORCINE RESPIRATORY CORONAVIRUS DIFFERS FROM TRANSMISSIBLE GASTROENTERITIS VIRUS BY A FEW GENOMIC DELETIONS
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DOI：
10.1099/0022-1317-71-11-2599
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发表时间：
1990-11-01
影响因子：
3.8
通讯作者：
LAUDE, H
中科院分区：
文献类型：
--
作者：
RASSCHAERT, D;DUARTE, M;LAUDE, H
对猪呼吸道冠状病毒（PRCV）的基因组组织进行了研究。PRCV是一种新发现的与肠致病性传染性胃肠炎病毒（TGEV）有密切抗原关系的病原体。从细胞培养的PRCV（法国分离物RM4）中获得基因组RNA，用于产生覆盖穗状(S)糖蛋白基因（7519个核苷酸）基因组3'末端到开始的cDNA克隆。鉴定出6个开放阅读框（orf），可翻译3个冠状病毒结构蛋白和3个假定的非结构多肽，与TGEV orf同源，命名为NS3-1、NS4和NS7。将PRCV的核苷酸和氨基酸序列与3株TGEV的序列数据进行两两比对，发现它们的总体同源性为96%。然而，PRCV的基因组表现出两个重要的显著特征。首先，S基因在5”区域缺少672个核苷酸，编码了S多肽的截断形式；其次，由于双缺失，S基因下游的第一个NS ORF被预测为无功能。讨论了基因组缺失对冠状病毒的组织趋向性和进化的意义。
The genome organization of porcine respiratory coronavirus (PRCV), a newly recognized agent which has a close antigenic relationship to the enteropathogenic transmissible gastroenteritis virus (TGEV), was studied. Genomic RNA from cell-cultured PRCV (French isolate RM4) was used to produce cDNA clones covering the genomic 3'' end to the start of the spike (S) glycoprotein gene (7519 nucleotides). Six open reading frames (ORFs) were identified that allowed the translation of three coronavirus structural proteins and three putative non-structural (NS) polypeptides, homologous to TGEV ORFs designated NS3-1, NS4 and NS7. Pairwise alignment of PRCV nucleotide and amino acid sequences with sequence data available for three TGEV strains revealed a 96% overall homology. However, the genome of PRCV exhibited two important distinctive features. The first was that the S gene lacked 672 nucleotides in the 5'' region and encoded a truncated form of the S polypeptide, and secondly, the first NS ORF downstream of the S gene was predicted to be non-functional as a consequence of a double deletion. The significance of genomic deletions with respect to tissue tropism and evolution of coronaviruses is discussed.