CRISPR/Cas9-mediated F1534S substitution in the voltage-gated sodium channel reveals its necessity and sufficiency for deltamethrin resistance in Aedes albopictus
CRISPR/Cas9-mediated F1534S substitution in the voltage-gated sodium channel reveals its necessity and sufficiency for deltamethrin resistance in Aedes albopictus
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DOI：
10.1007/s10340-022-01557-6
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发表时间：
2022-08-22
影响因子：
4.8
通讯作者：
Chen, Xiao-Guang
中科院分区：
文献类型：
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作者：
Guo, Yijia;Zhou, Jingni;Chen, Xiao-Guang
伊蚊对杀虫剂的抗药性是控制虫媒病毒疾病的主要挑战。然而，许多杀虫剂的抗药性机制仍不清楚。用常用杀虫剂溴氰菊酯筛选白纹伊蚊抗性品系，我们发现电压门控钠通道(VGSC)基因产物中的F1534S突变是产生抗性的第一事件。利用Cas9/gRNA技术设计F1534S替代，使以前敏感的品系对溴氰菊酯产生抗性。去除这种突变的杂交组合恢复了敏感的表型。预测的蛋白质结构变化和转录积累水平的差异与抗性表型相关。此外，在所有耐药Ae中均检测到F1534S突变。野外采集的白纹伊蚊种群。我们认为VGSC F1534S突变是Ae对溴氰菊酯产生抗性的重要原因。白纹伊蚊，是检测和监测拟除虫菊酯抗性的一个合适的分子指标。
Insecticide resistance in Aedes mosquitoes presents a major challenge to the control of arboviral diseases. However, resistance mechanisms for many of the insecticides remain unknown. A commonly used insecticide, deltamethrin, was used to select a resistance strain of the vector mosquito, Aedes albopictus, and we identified an F1534S substitution in the voltage-gated sodium channel (VGSC) gene product as the first event in generating resistance. Engineering an F1534S substitution using Cas9/gRNA technologies conferred deltamethrin resistance on a previously susceptible strain. Crosses that removed this mutation restored the susceptible phenotype. Predicted protein structural changes and differences in transcript accumulation levels were correlated with the resistance phenotype. Furthermore, F1534S mutations were detected in all resistant Ae. albopictus populations collected in the field. We conclude that the VGSC F1534S mutation is essential for resistance to deltamethrin in Ae. albopictus, and is a suitable molecular index for pyrethroid resistance detection and monitoring in this species.