Vitellogenin-immunohistochemistry in the Liver and the Testis of the Medaka, Oryzias latipes, Exposed to 17β-estradiol and p-nonylphenol
Vitellogenin-immunohistochemistry in the Liver and the Testis of the Medaka, Oryzias latipes, Exposed to 17β-estradiol and p-nonylphenol
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DOI：
10.2108/zsj.22.453
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发表时间：
2005-04
期刊：
影响因子：
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通讯作者：
Kayo Kobayashi;S. Tamotsu;K. Yasuda;T. Oishi
中科院分区：
文献类型：
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作者：
Kayo Kobayashi;S. Tamotsu;K. Yasuda;T. Oishi
摘要雄性鱼类产生的卵黄蛋白原（VTG）已被用作研究内分泌干扰物的生物标志物。然而，在雄鱼中产生的VTG的特性还没有得到很好的研究。本研究以17β-雌二醇（E2）和对壬基酚（NP）处理的青鳉（Oryzias latipes）为材料，研究了VTG在雄性青鳉肝脏和睾丸中的定位。将雄鱼暴露于1 μg/LE 2和500 μg/LNP中1-12 d。将对照组保持在仅包括媒介物的水中。用青鳉VTG抗血清作为第一抗体，对肝脏和睾丸的冷冻切片进行免疫组织化学染色。在E2和NP处理的肝脏中，肝细胞显示免疫反应性。特别是，靠近血窦周围细胞膜的细胞质呈强免疫阳性。在两种处理的睾丸中，间质组织和生精小管中的细胞（精母细胞）呈免疫阳性。随着处理时间的延长，两种组织中VTG的浓度逐渐升高。这些结果表明，睾丸中的生殖细胞与E2和NP处理能够纳入和积累VTG。
Abstract Vitellogenin (VTG) produced in male fish has been used for a biomarker to study endocrine disrupters. However, the characteristics of VTG produced in male fish have not been studied well. In this study, we investigated the localization of VTG in the liver and the testis of male medaka (Oryzias latipes) treated with 17β-estradiol (E2) and p-nonylphenol (NP). The male fish were exposed to 1 μg/L E2 and 500 μg/L NP for 1–12 days. Control groups were kept in water including only vehicle. The frozen sections of the liver and the testis were stained with immunohistochemical methods using an antiserum against medaka VTG as the first antibody. In the E2 and NP treated liver, the hepatocytes showed immunoreactivity. In particular, the cytoplasm close to the cell membrane surrounding the sinusoids was strongly immunopositive. In the testis of both treatments, the interstitial tissues and the cells (spermatocytes) in the seminiferous tubules were immunopositive. The concentration of VTG became gradually higher in both tissues with longer treatments. These results suggest that germ cells in the testis treated with E2 and NP are able to incorporate and accumulate VTG.