Expression of a functional antibody fragment in the gut of Rhodnius prolixus via transgenic bacterial symbiont Rhodococcus rhodnii
Expression of a functional antibody fragment in the gut of Rhodnius prolixus via transgenic bacterial symbiont Rhodococcus rhodnii
复制标题
DOI：
10.1046/j.1365-2915.1999.00175.x
复制
发表时间：
1999-05-01
影响因子：
1.9
通讯作者：
Beard, CB
中科院分区：
文献类型：
--
作者：
Durvasula, RV;Gumbs, A;Beard, CB
通过微生物共生体在昆虫体内表达外源抗寄生虫基因产物可用于防止媒介传播的病原体向脊椎动物宿主的传播。转基因共生菌Rhodococcus rhodnii表达的功能性抗体片段（rDB 3编码结合孕酮的鼠V-H/K）被输出到锥蝽Rhodnius prolixus（半翅目：猎蝽科）的肠腔中，该锥蝽是恰加斯病的载体。转基因共生体在Rhodnius prolixus的连续若虫龄和成虫中维持，尽管与天然未转化的Rhodococcus rhodnii竞争。这是第一次描述在昆虫中表达的功能性哺乳动物抗体片段。我们的系统是一个模型，用于构建paratransgenic昆虫（携带转化共生体的昆虫）与妥协的能力，传播病原体。
Expression within insects of foreign antiparasitic gene products via microbial symbionts could be used to prevent transmission of vector-borne pathogens to vertebrate hosts. Genetically transformed symbiotic bacteria Rhodococcus rhodnii expressed functional antibody fragments (rDB3 encoding murine V-H/K which binds progesterone) that were exported into the gut lumen of the triatomine bug Rhodnius prolixus (Hemiptera: Reduviidae), a vector of Chagas disease, Transgenic symbionts were maintained in successive nymphal instars and adults of Rhodnius prolixus despite competition with native untransformed Rhodococcus rhodnii. This is the first description of a functional mammalian antibody fragment expressed in an insect. Our system is a model for constructing paratransgenic insects (insects carrying transformed symbionts) with compromised ability to transmit pathogens.