High Efficiency DNA Transformation of Saccharomyces cerevisiae with the LiAc/SS-DNA/PEG Method

High Efficiency DNA Transformation of Saccharomyces cerevisiae with the LiAc/SS-DNA/PEG Method
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DOI:
10.1007/978-3-319-10142-2_17
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发表时间:
2015-01-01
期刊:
GENETIC TRANSFORMATION SYSTEMS IN FUNGI, VOL 1
影响因子:
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通讯作者:
Gietz, R. Daniel
Gietz, R. Daniel
中科院分区:
其他
文献类型:
--
作者:
Gietz, R. Daniel

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The ability to transform DNA into an organism is an essential element that aids greatly in many molecular and genetic studies. InSaccharomyces cerevisiaenumerous methods can be used to accomplish this task; however, the LiAc/ssDNA/PEG methods are presented here. Methods for various applications are listed including a method for transformation in 96 well microtiter plate format as well as a method for the production and transformation of frozen competent yeast cells.