Effects of triiodothyronine and propylthiouracil on plasma lipoproteins in male rats.
Effects of triiodothyronine and propylthiouracil on plasma lipoproteins in male rats.
复制标题
DOI：
--
复制
发表时间：
1982-11
影响因子：
6.5
通讯作者：
H. Wilcox;W. G. Keyes;T. Hale;R. Frank;D. W. Morgan;M. Heimberg
中科院分区：
文献类型：
--
作者：
H. Wilcox;W. G. Keyes;T. Hale;R. Frank;D. W. Morgan;M. Heimberg
在用三碘甲状腺原氨酸（T（3））处理7天的大鼠中观察到高脂蛋白血症，其特征是血浆apoA-I和HDL脂质和蛋白浓度升高。血浆HDL载脂蛋白的增加一般为apoC，apoE + A-IV，和apoA-I，通过等电聚焦测定。在甲状腺功能亢进状态下也观察到以VLDL和apoB浓度降低为特征的低胆固醇血症。虽然在轻度甲状腺功能减退动物（丙硫氧嘧啶治疗），游离脂肪酸的肝脏代谢转向酯化甘油三酯和VLDL的形成，正如我们以前报道的，血浆HDL和apoA-I浓度与对照血浆值没有差异，而d 1.006-1.063 g/ml（IDL + LDL）脂蛋白分数往往增加。在一般情况下，载脂蛋白E（IDL + LDL）部分的甲状腺功能减退大鼠的比例大于在控制和甲状腺功能亢进的动物，而载脂蛋白E的比例往往是较低的VLDL从甲状腺功能减退和甲亢大鼠比VLDL从控制。从T（3）处理的大鼠中分离的灌流肝脏中也观察到apoA-I的释放增加; apoA-I输出增加可能部分解释了在这些大鼠中观察到的高脂蛋白血症。用丙基硫氧嘧啶（PTU）处理的大鼠的灌注肝脏在体外抑制apoA-I的净输出，在完整动物中HDL或apoA-I的血浆浓度在统计学上显著降低时不表达。用T（3）处理也导致各种脂质类中必需脂肪酸含量的改变。T（3）处理大鼠血浆磷脂和酯化胆固醇中亚油酸残基显著减少，花生四烯酸含量增加。然而，未酯化脂肪酸和甘油三酯脂肪酸的相对脂肪酸组成没有被T（3）处理改变。PTU治疗对任何血脂中的脂肪酸分布均无影响。在T（3）处理的大鼠的灌注肝脏中，胆汁脂质的分泌增加，而PTU处理不影响胆汁中脂质的释放。这些观察结果表明甲状腺激素的调节作用，决定血浆HDL和其他脂蛋白的浓度和组成。Wilcox，H. G.，W. G. Keyes，T. A.黑尔河Frank，D. W. Morgan和M.海姆伯格三碘甲状腺原氨酸和丙基硫氧嘧啶对雄性大鼠血浆脂蛋白的影响。
Hyperalphalipoproteinemia, characterized by increased plasma concentrations of apoA-I and of HDL lipid and protein, was observed in rats treated with triiodothyronine (T(3)) for 7 days. The increase in the plasma HDL apoproteins was general for apoC, apoE plus A-IV, and apoA-I, as determined by isoelectric focusing. Hypotriglyceridemia, characterized by decreased concentrations of VLDL and apoB, was also observed in the hyperthyroid state. Although in the mildly hypothyroid animals (propylthiouracil-treated), hepatic metabolism of free fatty acid is shifted toward esterification to triglyceride and VLDL formation, as we reported previously, plasma HDL and apoA-I concentrations were not different from control plasma values, while the d 1.006-1.063 g/ml (IDL + LDL) lipoprotein fraction tended to be increased. In general, the proportion of apoE in the (IDL + LDL) fraction of the hypothyroid rat was greater than in controls and hyperthyroid animals, while the proportion of apoE tended to be lower in VLDL from both hypo- and hyperthyroid rats than in VLDL from controls. An enhanced release of apoA-I by perfused livers isolated from rats treated with T(3) was also observed; this enhanced output of apoA-I may explain, in part, the hyperalphalipoproteinemia observed in these rats. The depressed net output of apoA-I in vitro by perfused livers from rats treated with propylthiouracil (PTU) was not expressed in a statistically significant diminished plasma concentration of HDL or apoA-I in the intact animals. Treatment with T(3) also resulted in modification of the content of essential fatty acids in various lipid classes. Linoleic acid residues were significantly reduced and arachidonic acid content was increased in plasma phospholipids and esterified cholesterol in T(3)-treated rats. However, the relative fatty acid composition of unesterified fatty acids and triglyceride fatty acids was not altered by T(3) treatment. PTU treatment had no effect on fatty acid distribution in any of the plasma lipids. Secretion of biliary lipids was increased in perfused livers from T(3)-treated rats, while treatment with PTU did not affect release of lipids in the bile. These observations suggest a regulatory role for thyroid hormones that determine concentration and composition of plasma HDL and other lipoproteins.-Wilcox, H. G., W. G. Keyes, T. A. Hale, R. Frank, D. W. Morgan, and M. Heimberg. Effects of triiodothyronine and propylthiouracil on plasma lipoproteins in male rats.