THE ARREST OF REPLICATION FORKS IN THE RDNA OF YEAST OCCURS INDEPENDENTLY OF TRANSCRIPTION
THE ARREST OF REPLICATION FORKS IN THE RDNA OF YEAST OCCURS INDEPENDENTLY OF TRANSCRIPTION
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DOI：
10.1016/0092-8674(92)90355-g
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发表时间：
1992-10-16
期刊：
影响因子：
64.5
通讯作者：
FANGMAN, WL
中科院分区：
文献类型：
--
作者：
BREWER, BJ;LOCKSHON, D;FANGMAN, WL
复制叉与转录方向相反，在S.啤酒。由于它的位置和极性，我们测试的假设，这复制叉障碍（RFB）的结果从转录的行为。有三个结果与这一假设相矛盾。首先，RFB在含有RNA聚合酶I的135kd亚基的基因破坏的菌株中持续存在。第二，RFB在移植到质粒时引起复制叉的极性停滞。第三，RNA聚合酶II对缺少RFB的35S转录单位的质粒拷贝的转录不产生屏障。我们提出，通过将一种或多种蛋白质结合到RFB中的两个紧密间隔的位点，复制叉以定向方式被阻止。
Replication forks, moving opposite to the direction of transcription, are arrested at the 3' ends of the 35S transcription units in the rDNA locus of S. cerevisiae. Because of its position and polarity, we tested the hypothesis that this replication fork barrier (RFB) results from the act of transcription. Three results contradict this hypothesis. First, the RFB persists in a strain containing a disruption of the gene for the 135 kd subunit of RNA polymerase I. Second, the RFB causes a polar arrest of replication forks when transplanted to a plasmid. Third, transcription by RNA polymerase II of a plasmid copy of the 35S transcription unit lacking the RFB does not generate a barrier. We propose that replication forks are arrested in a directional manner through the binding of one or more proteins to two closely spaced sites in the RFB.