ICAM-1 A LIGAND FOR LFA-1-DEPENDENT ADHESION OF B-CELLS, T-CELLS AND MYELOID CELLS
ICAM-1 A LIGAND FOR LFA-1-DEPENDENT ADHESION OF B-CELLS, T-CELLS AND MYELOID CELLS
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DOI：
10.1038/331086a0
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发表时间：
1988-01-07
期刊：
影响因子：
64.8
通讯作者：
SHAW, S
中科院分区：
文献类型：
--
作者：
MAKGOBA, MW;SANDERS, ME;SHAW, S
细胞-细胞粘附对于许多免疫功能是必不可少的1 -4。LFA-1分子是粘附分子超家族的成员，参与粘附，这对白细胞的三个主要亚群中的每一个的功能至关重要：淋巴细胞、单核细胞和粒细胞5。在不同的实验室中，使用三种不同的单克隆抗体（在特定的模型系统中抑制LFA-1介导的白细胞粘附），已经在功能上鉴定了推定的LFA-1配体;然而，可能有一种以上的LFA-1配体6 -8。我们直接比较了三种相关的单克隆抗体，并表明每种抗体都结合相同的分子，细胞间粘附分子-1（ICAM-1）。最重要的是，B、T和髓样细胞特异性粘附于纯化的ICAM-1包被的表面;这种粘附对Mg 2+和Ca 2+具有不同的要求。这构成了ICAM-1作为LFA-1依赖性粘附的各种白细胞的配体的生化证据。
Cell-cell adhesion is essential for many immunological functions1–4. The LFA-1 molecule, a member of a superfamily of adhesion molecules, participates in adhesion which is critical to the function of each of the three major subsets of leukocytes: lymphocytes, monocytes and granulocytes5. Putative LFA-1 ligands have been identified functionally in different laboratories using three different monoclonal antibodies that inhibit LFA-1-mediated leukocyte adhesion in particular model systems; however, there may be more than one LFA-1 ligand6–8. We have directly compared the three relevant monoclonal antibodies, and show that each binds to the same molecule, intercellular-adhesion molecule-1 (ICAM-1). Most important, B, T and myeloid cells adhere specifically to purified ICAM-1-coated surfaces; such adhesion has distinctive requirements for Mg2+and Ca2+. This constitutes biochemical evidence that ICAM-1 functions as a ligand for LFA-1-dependent adhesion by a variety of leukocytes.