The efficacy of mesenchymal stem cells to regenerate and repair dental structures.
The efficacy of mesenchymal stem cells to regenerate and repair dental structures.
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DOI：
10.1111/j.1601-6343.2005.00331.x
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发表时间：
2005-08-01
影响因子：
3.1
通讯作者：
Gronthos, S
中科院分区：
文献类型：
--
作者：
Shi, S;Bartold, P M;Gronthos, S
目的：人牙组织来源的间充质干细胞的鉴定、鉴定及应用前景。方法：从正常人阻生第三磨牙中获取牙髓和牙周膜。将组织在胶原酶/分散酶中消化以产生单细胞悬浮液。将细胞在补充有20%胎牛血清、2 mM L-谷氨酰胺、100 μ M L-抗坏血酸-2-磷酸的α-MEM中培养。采用磁性和荧光激活细胞分选来表征新鲜分离和离体扩增的细胞群体的表型。在将羟基磷灰石/磷酸三钙（HA/TCP）颗粒共移植到免疫功能低下的小鼠体内8周后，评估培养细胞的发育潜力。成年人牙髓组织中存在MSC（牙髓干细胞，DPSC），人乳牙（来自人类脱落乳牙的干细胞，SHED）和牙周膜（牙周膜干细胞，PDLSC）通过它们在培养物中产生克隆形成细胞簇的能力而获得。体外扩增的DPSC、SHED和PDLSC群体表达与MSC、牙本质、骨、平滑肌、神经组织和内皮相关的标记物的异质分类。还发现PDLSC表达肌腱特异性标记物Scleraxis。含HA/TCP与DPSC或SHED的异种移植物产生了供体来源的牙本质牙髓样组织，其具有衬在矿化牙本质基质上的独特的成牙本质细胞层。在平行的研究中，PDLSC产生的牙骨质样结构与PDL样结缔组织移植时，HA/TCP到immunocomposedmice.CONCLUSION：总的来说，这些数据揭示了存在不同的MSC人口与牙齿结构的干细胞再生活的人牙组织在体内的潜力。
OBJECTIVES: Identification, characterization, and potential application of mesenchymal stem cells (MSC) derived from human dental tissues.METHODS: Dental pulp and periodontal ligament were obtained from normal human impacted third molars. The tissues were digested in collagenase/dispase to generate single cell suspensions. Cells were cultured in alpha-MEM supplemented with 20% fetal bovine serum, 2 mM l-glutamine, 100 microM l-ascorbate-2-phosphate. Magnetic and fluorescence activated cell sorting were employed to characterize the phenotype of freshly isolated and ex vivo expanded cell populations. The developmental potential of cultured cells was assessed following co-transplantation with hydroxyapetite/tricalcium phosphate (HA/TCP) particles into immunocompromised mice for 8 weeks.RESULTS: MSC were identified in adult human dental pulp (dental pulp stem cells, DPSC), human primary teeth (stem cells from human exfoliated deciduous teeth, SHED), and periodontal ligament (periodontal ligament stem cells, PDLSC) by their capacity to generate clongenic cell clusters in culture. Ex vivo expanded DPSC, SHED, and PDLSC populations expressed a heterogeneous assortment of makers associated with MSC, dentin, bone, smooth muscle, neural tissue, and endothelium. PDLSC were also found to express the tendon specific marker, Scleraxis. Xenogeneic transplants containing HA/TCP with either DPSC or SHED generated donor-derived dentin-pulp-like tissues with distinct odontoblast layers lining the mineralized dentin-matrix. In parallel studies, PDLSC generated cementum-like structures associated with PDL-like connective tissue when transplanted with HA/TCP into immunocompromised mice.CONCLUSION: Collectively, these data revealed the presence of distinct MSC populations associated with dental structures with the potential of stem cells to regenerate living human dental tissues in vivo.