Developmental regulation of galactoglycerolipid and galactosphingolipid sulphation during mammalian spermatogenesis. Evidence for a substrate-selective inhibitor of testicular sulphotransferase activity in the rat.
Developmental regulation of galactoglycerolipid and galactosphingolipid sulphation during mammalian spermatogenesis. Evidence for a substrate-selective inhibitor of testicular sulphotransferase activity in the rat.
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哺乳动物精子发生过程中半乳甘油脂和半乳鞘脂硫酸化的发育调节。
DOI：
10.1042/bj2310393
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Lingwood,CA
中科院分区：
文献类型：
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作者：
Lingwood,CA
硫酸氧半乳糖酰烷基甘油（SGG）的合成是哺乳动物精子发生的分化标志。出生后约20天，在大鼠睾丸中观察到最大硫酸化，并随着睾丸成熟迅速下降至低水平。目前的数据表明，SGG合成的这种下降是由于半乳糖脂硫酸化抑制剂的出现。抑制剂是一种可溶性睾丸因子，在出生后约25天首次检测到。睾丸匀浆在体外可硫酸化外源性半乳糖酰烷基甘油（GG）、半乳糖基神经酰胺（GC）和乳糖基神经酰胺（LC）。睾丸抑制剂在预防GG硫酸化方面最有效，在较小程度上抑制GC和LC硫酸化;这与以下发现相关：在睾丸发育的后期阶段，糖脂硫酸化从20日龄睾丸中的SGG生产转变为GC和LC硫酸化。还测定了抑制剂对脑和肾中磺基转移酶活性的影响。该抑制剂可减少睾丸和肾脏对体外GG的硫酸化作用，但对睾丸对GC的硫酸化作用的抑制作用较差，对肾脏和脑匀浆对GC的硫酸化作用无影响。一个9500倍的纯化的抑制活性已获得的馏分中分离的h.p.l.c.
The synthesis of sulphatoxygalactosylacylalkylglycerol (SGG) is a differentiation marker of mammalian spermatogenesis. Maximal sulphation is observed in rat testis at about 20 days after birth and rapidly declines to low levels as the testis matures. The present data show that this decline in SGG synthesis is due to the appearance of an inhibitor of galactolipid sulphation. The inhibitor is a soluble testicular factor which is first detected at about 25 days after birth. Testicular homogenate can sulphate exogenous galactosylacylalkylglycerol (GG), galactosylceramide (GC) and lactosylceramide (LC)in vitro. The testicular inhibitor is most effective in preventing GG sulphation and inhibits GC and LC sulphation to a lesser extent; this correlates with the finding that glycolipid sulphation shifts from SGG production in 20-day-old testis to GC and LC sulphation at later stages of testicular development. The effect of the inhibitor on sulphotransferase activity from brain and kidney was also determined. The inhibitor decreased the sulphation of GGin vitroby both testis and kidney, inhibited testicular sulphation of GC less effectively and had no effect on GC sulphation by kidney and brain homogenates. A 9500-fold purification of the inhibitory activity has been obtained in a fraction isolated by h.p.l.c.