HUMAN-IMMUNODEFICIENCY-VIRUS CONTAINS AN EPITOPE IMMUNOREACTIVE WITH THYMOSIN -ALPHA-1 AND THE 30-AMINO ACID SYNTHETIC P17 GROUP-SPECIFIC ANTIGEN PEPTIDE HGP-30
HUMAN-IMMUNODEFICIENCY-VIRUS CONTAINS AN EPITOPE IMMUNOREACTIVE WITH THYMOSIN -ALPHA-1 AND THE 30-AMINO ACID SYNTHETIC P17 GROUP-SPECIFIC ANTIGEN PEPTIDE HGP-30
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DOI：
10.1073/pnas.84.9.2951
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发表时间：
1987-05-01
影响因子：
11.1
通讯作者：
SARIN, PS
中科院分区：
文献类型：
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作者：
NAYLOR, PH;NAYLOR, CW;SARIN, PS
我们报道说，针对胸腺素制备的抗血清1 [与获得的免疫缺陷合成剂（AIDS）相关的人类免疫缺陷病毒共享同源性区域，有效地中和AIDS病毒，并防止了H9细胞在H9细胞中的复制。 。使用HPLC和免疫印迹分析，我们从克隆B，III型人类T-淋巴细胞病毒（HTLV-IIIB）中鉴定出一种蛋白质，其分子量为17,000的蛋白质，具有17,000的蛋白质，其抗胸腺素.alpha.Alpha.1。相反，在许多非人类物种的逆转录病毒提取物中未发现免疫反应性，包括猫，牛，猿猴，长臂猿和鼠逆转录病毒。针对30个氨基酸合成肽模拟（HGP-30）制备的异源抗血清不会与胸腺素交叉反应。Alpha.Alpha.1，但确实以与HTLV相同的方式与AIDS病毒的p17蛋白进行了特异性反应。 -IIIIB P17特异性单克隆抗体。这种合成类似物具有免疫原性和对HGP-30交叉反应的抗体不仅与合成肽，而且还与HTLV-IIIB p17病毒蛋白的抗体相关的抗体提供了证明艾滋病疫苗。此外，p17合成肽（HGP-30）可能被证明可用于诊断测定，可用于检测血清神经个体的艾滋病病毒感染。
We have reported that an antiserum prepared against thymosin .alpha.1 [which shares a region of homology with the p17 protein of the acquired immunodeficiency synthdrome (AIDS)-associated human immunodeficiency virus] effectively neutralized the AIDS virus and prevented its replication in H9 cells. Using HPLC and immunoblot analysis, we have identified from a clone B, type III human T-lymphotropic virus (HTLV-IIIB) extract a protein with a molecular weight of 17,000 that is immunoreactive with thymosin .alpha.1. In contrast, no immunoreactivity was found in retroviral extracts from a number of nonhuman species including feline, bovine, simian, gibbon, and murine retroviruses. Heterologous antiserum prepared against a 30-amino acid synthetic peptide analogue (HGP-30) does not cross-react with thymosin .alpha.1 but does react specifically with the p17 protein of the AIDS virus in a manner identical to that seen with an HTLV-IIIB p17-specific monoclonal antibody. The demonstration that this synthetic analogue is immunogenic and that antibodies to HGP-30 cross-react not only with the synthetic peptide but also with the HTLV-IIIB p17 viral protein provides an additional, and potentially more specific, candidate for development of a synthetic peptide vaccine for AIDS. In addition, the p17 synthetic peptide (HGP-30) may prove to be useful in a diagnostic assay for the detection of AIDS virus infection in seronegative individuals.