Background mutation in strain RB2126 affects the locomotion behavior of flp-1 mutants.
Background mutation in strain RB2126 affects the locomotion behavior of flp-1 mutants.
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背景突变影响Flp-1突变体的运动行为。
DOI：
10.17912/micropub.biology.000393
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发表时间：
2021-05-09
影响因子：
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通讯作者：
Topalidou I
中科院分区：
文献类型：
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作者：
Topalidou I
FLP-1神经肽参与运动、机械感觉和生殖。据报道，菌株RB2126含有flp-1(Ok2811)突变，这是一个潜在的零等位基因。在这里，我们报告了菌株RB2126，除了flp-1(Ok2811)外，还包含两个额外的背景突变。其中一个突变使动物获得了结构性达乌尔表型(Daf-c)，互补测试证实它位于Daf-c基因daf-11中。第二个突变是基于它部分抑制flp-1(Ok2811)突变体的体曲率升高这一事实而分离出来的，但它仍未被克隆。更新后的RB2126株基因为flp-1(Ok2811)；daf-11(Yak155)；yak156。
The FLP-1 neuropeptide is involved in locomotion, mechanosensation, and reproduction. Strain RB2126 is reported to contain the flp-1(ok2811) mutation, a prospective null allele. Here we report that strain RB2126, in addition to flp-1(ok2811), also contains two additional background mutations. One of the mutations gives the animals a constitutive dauer phenotype (Daf-c), and complementation testing confirms that it is in the Daf-c gene daf-11. The second mutation is isolated based on the fact that it partially suppresses the elevated body curvature of flp-1(ok2811) mutants, but it remains uncloned. The updated genotype of RB2126 strain is flp-1(ok2811); daf-11(yak155); yak156.