Optimizing Orthotopic Bladder Tumor Implantation in a Syngeneic Mouse Model
Optimizing Orthotopic Bladder Tumor Implantation in a Syngeneic Mouse Model
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DOI：
10.1016/j.juro.2009.08.020
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发表时间：
2009-12-01
影响因子：
6.6
通讯作者：
Larchian, William A.
中科院分区：
文献类型：
--
作者：
Chan, Eddie S. Y.;Patel, Amit R.;Larchian, William A.
目的：我们建立了一种在同系小鼠模型中原位植入膀胱肿瘤细胞的可靠技术。材料和方法：MBT-2 小鼠膀胱癌细胞经尿道植入同系 C3H/He 小鼠的膀胱（杰克逊实验室，巴港，缅因州）。肿瘤植入前使用不同的化学预处理，包括磷酸盐缓冲盐水（对照）、HCl、胰蛋白酶和聚-L-赖氨酸。化学预处理后将MBT-2细胞(1×10(6)或2×10(6))注入膀胱内空间。通过显微超声测定肿瘤体积和膀胱肿瘤体积。研究结束时收获膀胱，测量膀胱重量并进行组织病理学检查。结果：5只小鼠因明显不良反应而停止用HCl预处理膀胱，导致1只小鼠死亡，2只小鼠在预处理后3天出现严重膀胱炎症和血尿。磷酸盐缓冲盐水、胰蛋白酶和聚赖氨酸预处理6只动物，每只都耐受良好，没有明显不良反应或死亡。对照组、胰蛋白酶预处理组和聚赖氨酸预处理组的肿瘤取瘤率分别为33%、83%和83%。灌输 2 x 10(6) 细胞的小鼠的摄取率高于灌输 1 x 10(6) 细胞的小鼠（93% vs 73%，p < 0.05）。 结论：我们报告了一种可靠、可行的将膀胱肿瘤细胞原位植入同基因小鼠模型的方法。聚-L-赖氨酸和胰蛋白酶是有用的辅助预处理剂，可改善膀胱肿瘤的摄取。该模型可能适合评估膀胱癌的治疗范例。
Purpose: We established a reliable technique for orthotopically implanting bladder tumor cells in a syngeneic mouse model.Materials and Methods: MBT-2 murine bladder cancer cells were transurethrally implanted in the bladder of syngeneic C3H/He mice (Jackson Laboratory, Bar Harbor, Maine). Different chemical pretreatments were used before tumor implantation, including phosphate buffered saline (control), HCl, trypsin and poly-L-lysine. MBT-2 cells (1 x 10(6) or 2 x 10(6)) were instilled into the intravesical space after chemical pretreatment. Tumor take and bladder tumor volume were determined by micro ultrasound. Bladders were harvested at the end of the study to measure bladder weight and for histopathological examination.Results: Bladder pretreatment with HCl in 5 preparations was discontinued due to significant adverse reactions, resulting in death in 1 mouse, and severe bladder inflammation and hematuria 3 days after pretreatment in 2. Pretreatment with phosphate buffered saline, trypsin and poly-L-lysine in 6 animals each was tolerated well without significant adverse reactions or mortality. The tumor take rate in the control, trypsin and poly-L-lysine pretreatment groups was 33%, 83% and 83%, respectively. The take rate was higher in mice instilled with 2 x 10(6) cells than in those with 1 x 10(6) cells (93% vs 73%, p < 0.05).Conclusions: We report a reliable, feasible method of orthotopically implanting bladder tumor cells into a syngeneic mouse model. Poly-L-lysine and trypsin are useful adjunctive pretreatment agents to improve bladder tumor uptake. This model may be suitable to evaluate treatment paradigms for bladder cancer.