Induction of swimming in lamprey by L-DOPA and amino acids
Induction of swimming in lamprey by L-DOPA and amino acids
复制标题
L-多巴和氨基酸诱导七鳃鳗游泳
DOI：
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发表时间：
1980
期刊：
影响因子：
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通讯作者：
Margaret L. T. Poon
中科院分区：
文献类型：
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作者：
Margaret L. T. Poon
摘要：在洗浴液中加入多巴和酸性氨基酸，在小成年七鳃鳗（Ichthyomyzon unicuspis）离体肌层-脊髓制剂中产生游泳活性。身体的波动运动，对侧肌节的反相脉冲，以及脉冲所占周期的比例与完整动物的游泳行为相同。在分离的制剂中，肌瘤活动也表现出直立-尾侧相滞后。用抑制多巴胺和去甲肾上腺素合成或分解的药物处理后，左旋多巴的兴奋作用没有改变，儿茶酚胺本身也没有兴奋游泳活动。谷氨酸二乙酯竞争性地拮抗左旋多巴和酸性氨基酸的兴奋作用。士的宁、高K+和低Ca++在脊髓中产生一般兴奋，但不产生游泳活动。由此可见，左旋多巴对七鳃鳗脊髓神经元的刺激作用与酸性氨基酸类似。
SummaryL-DOPA and acidic amino acids, when applied in the bathing fluid, produced swimming activities in isolated myotome-spinal cord preparations of small adult lampreys (Ichthyomyzon unicuspis). Undulatory movements of the body, antiphasic bursts of spikes in contralateral myotomal segments, and the fraction of the cycle period occupied by the bursts were identical to those of swimming behavior in intact animals. In isolated preparations myotomal activities also exhibited rostro-caudal phase lag.The excitatory action of L-DOPA was not altered after treatment of the preparations with drugs which inhibit the synthesis or breakdown of dopamine and noradrenaline, nor did the catecholamines themselves excite swimming activity. Glutamic acid diethyl ester antagonized competitively the excitatory actions both of L-DOPA and of the acidic amino acids.Strychnine, high K+, and low Ca++ produced general excitation in the spinal cord but not swimming activities.It is concluded that L-DOPA excites neurons in the lamprey spinal cord by an action similar to that of acidic amino acids.