Simplified typing of mouse hemoglobin (Hbb) phenotypes using cystamine
Simplified typing of mouse hemoglobin (Hbb) phenotypes using cystamine
复制标题
使用胱胺对小鼠血红蛋白 (Hbb) 表型进行简化分型
DOI：
10.1007/bf00484723
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发表时间：
1978
影响因子：
2.4
通讯作者：
J. B. WhitneyIII
中科院分区：
文献类型：
--
作者：
J. B. WhitneyIII
用二硫化物试剂胱胺修饰的小鼠血红蛋白的醋酸纤维素电泳可以快速、明确地区分共显性小鼠血红蛋白“单一”（Hbb s ）和“扩散”（Hbb d 和 Hbb p ）等位基因的所有组合。单一、弥散性大、弥散性d-小和弥散性p-小成人血红蛋白都可以通过这种方法解析，这取决于“弥散”小鼠的β链中是否存在半胱氨酸，而“单一”小鼠的β链中没有发现半胱氨酸。
Cellulose acetate electrophoresis of mouse hemoglobins modified with the disulfide reagent cystamine permits rapid, unequivocal discrimination of all combinations of the codominant mouse hemoglobin “single” (Hbb s ) and “diffuse” (Hbb d and Hbb p ) alleles. The single, diffuse major, diffuse d-minor, and diffuse p-minor adult hemoglobins are all resolved by this method, which depends on the presence of a cysteine in the β chains of “diffuse” mice which is not found in the β chain of “single” mice.