CONTROL OF INFLUENZA-VIRUS GENE-EXPRESSION - QUANTITATIVE-ANALYSIS OF EACH VIRAL-RNA SPECIES IN INFECTED-CELLS
CONTROL OF INFLUENZA-VIRUS GENE-EXPRESSION - QUANTITATIVE-ANALYSIS OF EACH VIRAL-RNA SPECIES IN INFECTED-CELLS
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DOI：
10.1093/oxfordjournals.jbchem.a122702
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发表时间：
1989-04-01
影响因子：
2.7
通讯作者：
FUKUDA, R
中科院分区：
文献类型：
--
作者：
HATADA, E;HASEGAWA, M;FUKUDA, R
我们建立了一个定量杂交系统，分别测定了8个基因组片段的3种流感病毒rna （vRNA、mRNA和cRNA）。作为杂交探针，采用SP-6转录系统产生32p标记的正负极性RNA，并大量过量使用，足以克服病毒RNA样品中存在的互补RNA。利用该系统研究了A/Udorn/72 （H3N2）感染MDCK细胞后各病毒RNA种合成的控制情况。我们的新观察结果如下。1)在初级转录中观察到片段特异性转录。2)所有片段的病毒基因组复制同时开始。在携带晚期基因的片段的复制中没有观察到延迟。3)除了在转录水平上的调控外，病毒晚期基因的表达在转录后的一些步骤上也受到调控。这些结果与先前报道的概念不相容，并使我们提出了对病毒晚期基因表达的独特调控。
We established a quantitative hybridization system by which three types of influenza virus RNAs (vRNA, mRNA, and cRNA) for the 8 genome segments were measured individually. As the hybridization probes, 32P-labeled RNAs of both plus and minus polarity were produced employig an SP-6 transcription system and used in a large molar excess, sufficient to overcome complementary RNAs present in the viral RNA samples. Employing the system, we studied the control of the synthesis of each viral RNA species in MDCK cells infected with A/Udorn/72 (H3N2). Our new observations were as follows. 1) Segment-specific transcription was observed at the primary transcription. 2) Replication of the virus genome began simultaneously for all segments. No delay was observed in the replication of the segments carrying late genes. 3) In addition to control at the transcriptional levels, the expression of viral late genes was regulated at some post-transcriptional step(s). These results are not compatible with the concepts reported previously, and lead us to propose unique regulations operating on the expression of the viral late genes.