RELATIONSHIP BETWEEN ASTROCYTIC PROCESSES AND PERINEURONAL NETS IN RAT NEOCORTEX
RELATIONSHIP BETWEEN ASTROCYTIC PROCESSES AND PERINEURONAL NETS IN RAT NEOCORTEX
复制标题
DOI：
10.1002/glia.440150205
复制
发表时间：
1995-10-01
期刊：
影响因子：
6.2
通讯作者：
CELIO, MR
中科院分区：
文献类型：
--
作者：
BLUMCKE, I;EGGLI, P;CELIO, MR
“神经元周围网”（PNs）包裹着哺乳动物新皮层中一种抑制性神经元。根据PNs由神经胶质过程组成的说法，我们分析了大鼠新皮层细胞内注射的神经胶质细胞与PNs之间的关系。将路西法黄离子化注射到神经胶质细胞中，用绒毛草凝集素观察神经网络。使用激光共聚焦扫描显微镜，胶质突起和pn被确定为不同的结构。凝集素标记始终与细胞外空间有关，介于ly标记的星形胶质细胞突和神经元之间。在注射的不同类型胶质细胞中，只有密集分枝的原生质星形胶质细胞延长了可以追溯到接触PNs的过程。这些原生质星形胶质细胞也向邻近的未被PNs包裹的神经元和毛细血管发送过程。目前的数据有力地表明，PNs不是由胶质过程组成，而是支持PNs代表一些抑制性中间神经元和星形细胞过程表面之间的特殊细胞外物质的观点。(C) 1995 Wiley-Liss, Inc。
''Perineuronal nets'' (PNs) ensheath a subtype of inhibitory neurons in the mammalian neocortex. In the light of the proposal that PNs consist of glial processes, we have analyzed the relationship between intracellularly injected glial cells and PNs in the rat neocortex. Glial cells were injected iontophoretically with Lucifer Yellow in lightly fixed tissue slices and PNs were visualized with the lectin from Vicia villosa. Using confocal laser scanning microscopy, glial processes and PNs were identified as distinct structures. Lectin labeling was consistently associated with the extracellular space interposed between LY-labeled astrocyte processes and neurons. Of the different types of glial cells injected, only the densely-ramifying protoplasmic astrocytes extended processes which could be traced to contact PNs. These protoplasmic astrocytes also sent out processes to adjacent neurons not ensheathed by PNs, and to capillaries. The present data strongly suggests that PNs do not consist of glial processes but rather support the idea that PNs represent specialized extracellular material interposed between the surface of some inhibitory interneurons and astrocytic processes. (C) 1995 Wiley-Liss, Inc.