Premixed rapid-setting calcium phosphate composites for bone repair
Premixed rapid-setting calcium phosphate composites for bone repair
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DOI：
10.1016/j.biomaterials.2005.01.015
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发表时间：
2005-08-01
期刊：
影响因子：
14
通讯作者：
Chow, LC
中科院分区：
文献类型：
--
作者：
Carey, LE;Xu, HHK;Chow, LC
尽管磷酸钙骨水泥（CPC）在骨修复方面很有前景，但其临床应用需要现场粉末液体混合。为了缩短手术时间和改善移植物的性能，需要开发预混合CPC，其中膏体在储存期间保持稳定，只有在植入缺损后才会变硬。本研究的目的是开发浸在生理溶液中快速定型的预混CPC。预混CPC的配制方法如下：预混CPC = CPC粉末+非水液体+胶凝剂+硬化促进剂。制备了三种预混合的聚氯乙酸：聚氯乙酸-一水磷酸钙（MCPM）、聚氯乙酸-壳聚糖和聚氯乙酸-酒石石。这些新的预混CPC的凝固时间从5.3到7.9分钟不等，显著快于之前报道的预混对照CPC的61.7分钟（p < 0.05）。扫描电镜显示，羟基磷灰石浸泡1d后形成针状纳米羟基磷灰石晶体，浸泡7d后晶体生长。预混合CPCs在7d时的直径抗拉强度范围为2.8至6.4MPa，与报道的松质骨和烧结多孔羟基磷灰石植入物的强度相当。成骨细胞在CPC-MCPM和cpc -壳聚糖上获得正常的多边形形态，细胞质延伸粘附在纳米羟基磷灰石晶体上。综上所述，为了避免手术中粉末与液体的混合，开发了快速凝固的预混CPCs。浆料一旦浸入生理溶液中迅速硬化，形成羟基磷灰石。骨水泥具有与松质骨和烧结多孔羟基磷灰石相匹配的强度，并且与传统的非预混CPC相似，没有细胞毒性。(c) 2005 Elsevier Ltd版权所有。
Although calcium phosphate cement (CPC) is promising for bone repair, its clinical use requires on site powder liquid mixing. To shorten surgical time and improve graft properties, it is desirable to develop premixed CPC in which the paste remains stable during storage and hardens only after placement into the defect. The objective of this study was to develop premixed CPC with rapid setting when immersed in a physiological solution. Premixed CPCs were formulated using the following approach: Premixed CPC = CPC powder + nonaqueous liquid + gelling agent + hardening accelerator. Three premixed CPCs were developed: CPC-monocalcium phosphate monohydrate (MCPM), CPC-chitosan, and CPC-tartaric. Setting time for these new premixed CPCs ranged from 5.3 to 7.9 min, significantly faster than 61.7 min for a premixed control CPC reported previously (p < 0.05). SEM revealed the formation of nano-sized needle-like hydroxyapatite crystals after 1d immersion and crystal growth after 7d. Diametral tensile strength for premixed CPCs at 7d ranged from 2.8 to 6.4MPa, comparable to reported strengths for cancellous bone and sintered porous hydroxyapatite implants. Osteoblast cells attained a normal polygonal morphology on CPC-MCPM and CPC-chitosan with cytoplasmic extensions adhering to the nano-hydroxyapatite crystals. In summary, fast-setting premixed CPCs were developed to avoid the powder liquid mixing in surgery. The pastes hardened rapidly once immersed in physiological solution and formed hydroxyapatite. The cements had strengths matching those of cancellous bone and sintered porous hydroxyapatite and noncytotoxicity similar to conventional non-premixed CPC. (c) 2005 Elsevier Ltd. All rights reserved.