Number and function of circulating human antigen presenting cells regulated by sleep
Number and function of circulating human antigen presenting cells regulated by sleep
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DOI：
10.1093/sleep/30.4.401
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发表时间：
2007-04-01
期刊：
影响因子：
5.6
通讯作者：
Born, Jan
中科院分区：
文献类型：
--
作者：
Dimitrov, Stoyan;Lange, Tanja;Born, Jan
研究目的：有证据表明，睡眠促进了对传染性病原体的适应性免疫反应，从而支持免疫记忆。这种效果可能是通过睡眠引起的树突状细胞（dc）数量和功能的变化来实现的，树突状细胞在免疫反应的启动中起着关键作用。本研究旨在分离睡眠和昼夜节律对DC前体循环数量的影响，即CD14(+)CD16（-）和CD14(dim)CD16（+）单核细胞、髓样树突状细胞前体（pre-mDC）和浆细胞样树突状细胞（PDC），以及这些细胞产生的2个关键细胞因子，即白细胞介素(IL)-12和干扰素(IFN)- α。设计：在受试者内交叉设计中，人类受试者在2种情况下进行检查，即在正常的睡眠-觉醒周期和24小时清醒期间。夜间每1.5小时取一次血，白天每3小时取一次血。设置：实验在受控的实验室条件下进行。参与者：27名年龄在18至30岁之间的健康男性。测量和结果：与清醒相比，睡眠与mdc前产生IL-12的数量显著增加有关，IL-12是Th1反应的主要诱导剂。此外，睡眠会略微降低PDC和T细胞计数，但不影响PDC产生ifn - α。然而，睡眠大大减少了CD14（弱）CD16（+）单核细胞的数量，这可能反映了与睡眠相关的儿茶酚胺释放下降导致细胞边缘增加。结论：我们的数据确定了mdc前产生IL-12是睡眠的基本目标，与成熟APC功能最密切相关，因此睡眠可以有效增强适应性免疫反应。
Study Objectives: There is evidence that sleep facilitates the adaptive immune response to infectious agents and, thereby, supports immunologic memory. The effect might be attained by sleep-induced changes in the number and function of dendritic cells (DCs), which play a key role in the initiation of the immune response. This study aimed to dissociate effects of sleep and circadian rhythm on circulating numbers of DC precursors, ie, CD14(+)CD16(-) and CD14(dim)CD16(+) monocytes, myeloid dendritic cell precursors (pre-mDC), and plasmacytoid dendritic cells (PDC) and on 2 key cytokines produced by these cells, ie, interleukin (IL)-12 and interferon (IFN)-alpha.Design: In a within-subject cross-over design, human subjects were examined on 2 occasions, ie, during a normal sleep-wake cycle and during 24 hours of wakefulness. Blood was sampled every 1.5 hours during nighttime and every 3 hours during daytime.Setting: Experiments took place under controlled laboratory conditions.Participants: Twenty-seven healthy men aged between 18 and 30 years.Measurements and Results: Compared with wakefulness, sleep was associated with a striking increase in the number of pre-mDC producing IL-12, which is a main inducer of Th1 responses. In addition, sleep slightly decreased PDC and also T cell counts but did not affect IFN-alpha production by PDC. Sleep, however, substantially decreased numbers of CD14(dim)CD16(+) monocytes, probably reflecting increased margination of the cells upon a sleep-related drop in catecholamine release.Conclusions: Our data identify pre-mDC producing IL-12 as a basic target of sleep that is most closely related to mature APC function and whereby sleep can effectively enhance adaptive immune responses.