CD36-dependent adhesion and knob expression of the transmission stages of Plasmodium falciparum is stage-specific
CD36-dependent adhesion and knob expression of the transmission stages of Plasmodium falciparum is stage-specific
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DOI：
10.1016/s0166-6851(98)00040-1
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发表时间：
1998-06-01
影响因子：
1.5
通讯作者：
Culvenor, JG
中科院分区：
文献类型：
--
作者：
Day, KP;Hayward, RE;Culvenor, JG
恶性疟原虫滋养体通过与宿主内皮细胞的黏附从外周循环中隔离。配子体，在成熟过程中也会隔离。通过感染细胞与C32黑色素瘤细胞(C32MC)的结合以及纯化的黏附蛋白、白细胞分化抗原(CD36)和细胞间黏附分子-1(ICAM-1)来分析几个分离/克隆的I-V期配子体的黏附表型。这些细胞和蛋白质此前已被证明是滋养体黏附的受体。早期配子细胞(I-IIA期)与C32MC和纯化的受体CD36结合，但不与ICAM-1结合。早期配子细胞通过CD36与C32MC结合，参与这一结合的寄生虫配体是胰酶敏感的。IIB-V期配子体与C32MC、CD36和ICAM-1均无粘附性。被称为纽结的电子致密突起和富含组氨酸的蛋白1(HRP 1)的表达与滋养体与CD36的黏附有关。早期感染配子体的细胞表面有结节，而晚期感染的配子体细胞表面没有结节。HRP-1表达的阶段特异性模式也被观察到，这与该分子在早期配子细胞CD36黏附中的作用一致。这些年轻配子体的黏附表型与滋养体的黏附表型在所有标准上都是无法区分的。这些数据支持其他宿主受体介导晚期配子体结合的假说。(C)1998 Elsevier Science B.V.保留所有权利。
Plasmodium falciparum trophozoites sequester from the peripheral circulation by adherence to host endothelium. Gametocytes, also sequester during maturation. Analysis of the adhesion phenotype of stage I to V gametocytes of several isolates/clones was assessed by binding of infected cells to C32 melanoma cells (C32MC) and the purified adhesion proteins, leucocyte differentiation antigen (CD36) and intercellular adhesion molecule-1 (ICAM-1). These cells and proteins, have previously been shown to be receptors for adherence of trophozoites. Early gametocytes (stages I-IIA) were found to bind to C32MC as well as the purified receptor CD36 but not to ICAM-1. Early gametocytes bound to C32MC via CD36 and the parasite ligand involved in this binding was trypsin sensitive. Stage IIB to V gametocytes did not adhere to C32MC, CD36 nor ICAM-1. Electron-dense protruberances known as knobs and histidine rich protein 1 (HRP 1) expression have been associated with trophozite adhesion to CD36. Knobs were present at the surface of early but not late gametocyte infected cells. Stage-specific patterns of HRP 1 expression, consistent with a role for this molecule in CD36 adhesion of early gametocytes, were also observed. The adhesion phenotype of these young gametocytes was indistinguishable from that of the trophozoites by all criteria examined. These data support the hypothesis that other host receptors mediate the binding of late gametocytes. (C) 1998 Elsevier Science B.V. All rights reserved.