Donor nerve axotomy and axonal regeneration after end-to-side neurorrhaphy in a rodent model.
Donor nerve axotomy and axonal regeneration after end-to-side neurorrhaphy in a rodent model.
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DOI：
10.3171/2017.8.jns17739
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发表时间：
2018-02
影响因子：
4.1
通讯作者：
Shiwei Wang;Diya Su;Jing Li;De-zhi Li;H. Wan;M. Schumacher;Song Liu
中科院分区：
文献类型：
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作者：
Shiwei Wang;Diya Su;Jing Li;De-zhi Li;H. Wan;M. Schumacher;Song Liu
目的在这项研究中，作者使用成年大鼠神经移植物和供体胫神经之间的端侧神经缝合手术模型来研究供体神经诱导轴突再生的最佳条件。他们还评估了使用预退化神经移植物吸引再生轴突重新生长到移植物中，然后引导和改善其向目标方向生长的更有利途径的重要性，与新鲜神经移植物获得的结果相比。方法在神经移植物和供体胫神经之间进行端侧神经缝合术。神经移植物取自左侧胫神经，该神经在神经缝合前 1 周被新鲜切除或预退化。供体右胫神经仅因神经外膜去除而损伤，因神经外膜去除而损伤接合部位总轴突的20％或50％的横截面，或保持完整。术后随访6周，通过光学显微镜和逆行标记评估结果，以检测位于腰椎背根神经节的再生初级感觉神经元和位于腰椎腹角的脊髓运动神经元。 结果在随访期结束时，当供体神经保持完整时，在神经移植物中未观察到再生轴突，而当供体神经因神经外膜去除而损伤时，仅检测到很少的轴突。独自一人。然而，当供体神经被轴突切断时，移植物中出现了许多再生轴突，并且50％横截面轴突切断的神经达到了最大数量。在具有 50% 供体神经横截面的大鼠中，观察到移植物具有更好的神经样形态，没有结缔粘连。当使用预退化的神经移植物时，更多的再生轴突被吸引并拉长，形状更规则，髓鞘形成得到改善。结论神经移植物中轴突的再生取决于端侧神经缝合后供体神经的轴突切除术。当使用预退化的神经移植物时，可以实现更有效的吸引和改善的再生轴突结构。此外，神经移植物可能需要一定数量的再生轴突以维持神经样形态。
OBJECTIVEIn this study, the authors used a surgical model of end-to-side neurorrhaphy between a nerve graft and a donor tibial nerve in adult rats to investigate the optimal conditions for axonal regeneration induced by the donor nerve. They also assessed the importance of a more favorable pathway using a predegenerated nerve graft to attract regenerating axons to regrow into the graft and then directing and improving their growth toward the target in comparison with results obtained with a fresh nerve graft.METHODSEnd-to-side neurorrhaphy was performed between a nerve graft and a donor tibial nerve. The nerve graft was obtained from the left tibial nerve, which was either freshly removed or predegenerated 1 week prior to neurorrhaphy. The donor right tibial nerve was injured by epineurium removal alone, injured by epineurium removal with cross section of 20% or 50% of the total axons at the coaptation site, or left intact. The animals were followed postoperatively for a 6-week period, and outcomes were evaluated by optical microscopy and retrograde labeling to detect the regenerated primary sensory neurons located in the lumbar dorsal root ganglia and spinal motor neurons located in the lumbar spinal ventral horn.RESULTSAt the end of the follow-up period, no regenerating axons were observed in the nerve grafts when the donor nerve was left intact, and very few axons were detected when the donor nerve was injured by epineurium removal alone. However, numerous regenerating axons appeared in the grafts when the donor nerve was axotomized, and the greatest number was achieved with a 50% cross section axotomized nerve. In the rats with a 50% cross section of the donor nerve, better nerve-like morphology of the grafts was observed, without connective adhesions. When a predegenerated nerve graft was used, more regenerating axons were attracted and elongated with a more regular shape and improved myelination.CONCLUSIONSAxonal regrowth into a nerve graft depends on axotomy of the donor nerve after end-to-side neurorrhaphy. More efficient attraction and an improved structure of the regenerating axons were achieved when a predegenerated nerve graft was used. Furthermore, a nerve graft may require a certain number of regenerating axons to maintain a nerve-like morphology.