A novel murine model of oral candidiasis with local symptoms characteristic of oral thrush
A novel murine model of oral candidiasis with local symptoms characteristic of oral thrush
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DOI：
10.1111/j.1348-0421.2003.tb03403.x
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发表时间：
2003-01-01
影响因子：
2.6
通讯作者：
Abe, S
中科院分区：
文献类型：
--
作者：
Takakura, N;Sato, Y;Abe, S
本研究以小鼠口腔念珠菌病模型为研究对象，采用镇静剂建立了一种既具有口腔内稳定酵母菌群，又具有鹅口疮特征性症状的小鼠口腔念珠菌病模型。小鼠用泼尼松龙进行免疫抑制，并给予盐酸四环素。通过棉拭子用10（6）个白色念珠菌活细胞经口感染，并注射足够的氯化氯丙嗪，使其在接种后保持镇静状态约3小时。从接种后第3天至第7天，从每只小鼠的口腔中回收到10（5）-10（6）个念珠菌菌落形成单位，在舌头的大部分部位观察到发白的凝乳样斑块。显微镜下可见舌面出现芽管。该模型为研究口腔念珠菌病的发病机制提供了一种有效的实验手段。白念珠菌口腔感染和各种抗真菌剂的疗效微生物学和微生物。
A conventional and easy method to establish a murine oral candidiasis model, which has not only a stable yeast population in the oral cavity but also symptoms characteristic of oral thrush, was developed by using a sedative agent. Mice were immunosuppressed with prednisolone and were given tetracycline hydrochloride. They were orally infected with 10(6) viable cells of Candida albicans by means of a cotton swab and enough chlorpromazine chloride had been injected to keep them in a sedative state about for 3 hr after inoculation. From day 3 to day 7 post inoculation, 10(5)-10(6) colony forming units of Candida were recovered from the oral cavity of each mouse and whitish, curd-like patches were observed on most parts of tongue. Microscopically, germ tubes had appeared on the tongue surface. This model would be a useful experimental oral candidiasis for investigating the pathogenesis of C. albicans oral infection and the efficacy of various antifungal agents microbiologically and symptomatically.