BORRELIA-BURGDORFERI IS CLONAL - IMPLICATIONS FOR TAXONOMY AND VACCINE DEVELOPMENT
BORRELIA-BURGDORFERI IS CLONAL - IMPLICATIONS FOR TAXONOMY AND VACCINE DEVELOPMENT
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DOI：
10.1073/pnas.90.21.10163
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发表时间：
1993-11-01
影响因子：
11.1
通讯作者：
LUFT, BJ
中科院分区：
文献类型：
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作者：
DYKHUIZEN, DE;POLIN, DS;LUFT, BJ
对15株莱姆病螺旋体的染色体基因弗拉和p93以及来自线性质粒的ospA基因进行了测序，基因树的比较没有提供染色体基因之间遗传交换的证据，提示为B。burgdorferi是严格无性系的。染色体基因树与质粒编码的ospA的比较表明，克隆之间的质粒转移是罕见的。基因内重组的证据仅在单个ospA等位基因中发现。分析揭示了三种常见的克隆和一些罕见的克隆，这些克隆是如此的高度分化，以至于针对其中一种克隆开发的疫苗不太可能为其他生物体提供免疫力。因此，了解B的地理和遗传变异。burgdorferi将被证明对开发有效的疫苗和控制方案至关重要。虽然主要的克隆可能被视为不同的物种，克隆种群结构，地理位置，以及莱姆病的广泛发病率表明，B。burgdorferi应该仍然是整个生物体的名称。
The chromosomal genes fla and p93 and the ospA gene from a linear plasmid were sequenced from up to 15 isolates of Borrelia burgdorferi, which causes Lyme borreliosis in man. Comparison of the gene trees provides no evidence for genetic exchange between chromosomal genes, suggesting B. burgdorferi is strictly clonal. Comparison of the chromosomal gene trees with that of the plasmid-encoded ospA reveals that plasmid transfer between clones is rare. Evidence for intragenic recombination was found in only a single ospA allele. The analysis reveals three common clones and a number of rare clones that are so highly divergent that vaccines developed against one are unlikely to provide immunity to organisms from others. Consequently, an understanding of the geographic and genetic variability of B. burgdorferi will prove essential for the development of effective vaccines and programs for control. While the major clones might be regarded as different species, the clonal population structure, the geographic localization, and the widespread incidence of Lyme disease suggest that B. burgdorferi should remain the name for the entire array of organisms.