Proteome-Wide Analysis of Cysteine Reactivity during Effector-Triggered Immunity.
Proteome-Wide Analysis of Cysteine Reactivity during Effector-Triggered Immunity.
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效应器触发免疫过程中半胱氨酸反应性的全蛋白质组分析。
DOI：
10.1104/pp.18.01194
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发表时间：
2019
期刊：
影响因子：
7.4
通讯作者：
Popescu,SorinaC
中科院分区：
文献类型：
--
作者：
McConnell,EvanW;Berg,Philip;Westlake,TimothyJ;Wilson,KatherineM;Popescu,GeorgeV;Hicks,LeslieM;Popescu,SorinaC
在植物感染病原体和激活效应触发免疫（ETI）的早期阶段，氧化剂积累的激增产生细胞氧化还原电位的变化。氧化还原体是由氧化剂引起的蛋白质在蛋白质组范围内的氧化修饰，对后生动物的应激反应具有众所周知的影响。然而，植物免疫反应期间蛋白质和对氧化敏感的残基的身份在很大程度上仍然未知。以前对thimet寡肽酶TOP 1和TOP 2的研究将它们置于ETI的水杨酸依赖性分支中，目前的模型中TOP维持相互连接的细胞器和胞质途径，其调节氧化爆发和细胞死亡的发展。在这里，我们的特点是ETI氧化还原体在拟南芥（拟南芥）野生型Col-0和top1 top2突变体植物使用差分烷基化为基础的富集技术，结合无标记的质谱定量。我们在病原体感染后的多个时间点在广泛的蛋白质家族中鉴定了对氧化敏感的半胱氨酸。Col-0 andtop 1 top2氧化还原体之间的差异被检测到的身份和数量的氧化半胱氨酸，和蛋白质氧化的时间依赖性波动的幅度。我们的研究结果支持了决定性的作用，TOP在保持适当的水平和动态的蛋白质组氧化过程中ETI。这项研究显着扩大了对氧化敏感的植物蛋白的剧目，并可以指导未来的机制研究。
A surge in the accumulation of oxidants generates shifts in the cellular redox potential during early stages of plant infection with pathogens and activation of effector-triggered immunity (ETI). The redoxome, defined as the proteome-wide oxidative modifications of proteins caused by oxidants, has a well-known impact on stress responses in metazoans. However, the identity of proteins and the residues sensitive to oxidation during the plant immune response remain largely unknown. Previous studies of the thimet oligopeptidases TOP1 and TOP2 placed them in the salicylic acid dependent branch of ETI, with a current model wherein TOPs sustain interconnected organellar and cytosolic pathways that modulate the oxidative burst and development of cell death. Herein, we characterized the ETI redoxomes in Arabidopsis (Arabidopsis thaliana) wild-type Col-0 andtop1top2mutant plants using a differential alkylation-based enrichment technique coupled with label-free mass spectrometry-based quantification. We identified cysteines sensitive to oxidation in a wide range of protein families at multiple time points after pathogen infection. Differences were detected between Col-0 andtop1top2redoxomes regarding the identity and number of oxidized cysteines, and the amplitude of time-dependent fluctuations in protein oxidation. Our results support a determining role for TOPs in maintaining the proper level and dynamics of proteome oxidation during ETI. This study significantly expands the repertoire of oxidation-sensitive plant proteins and can guide future mechanistic studies.