Peony root extract upregulates transthyretin and phosphoglycerate mutase in mouse cobalt focus seizure
Peony root extract upregulates transthyretin and phosphoglycerate mutase in mouse cobalt focus seizure
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DOI：
10.1016/j.bbrc.2008.04.094
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发表时间：
2008-07-04
影响因子：
3.1
通讯作者：
Kimura, Minoru
中科院分区：
文献类型：
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作者：
Kajiwara, Kagemasa;Sunaga, Katsuyoshi;Kimura, Minoru
钴灶是一种癫痫灶模型，其中大脑神经元表现出长期严重的棘波放电，随后是神经元死亡。然而，当在钴应用之前施用牡丹根提取物（PR）时，神经元死亡被防止。我们测试的假设，PR调制的神经保护蛋白的表达在小鼠钴焦点的大脑蛋白质组学分析，使用二维聚丙烯酰胺凝胶电泳和质谱筛选差异表达的蛋白质。分析表明，甲状腺素运载蛋白，甲状腺激素和类维生素A的载体蛋白，和磷酸甘油酸酯β，糖酵解酶的大脑形式，在钴处理的小鼠大脑中上调，并进一步增加PR管理与上调的神经颗粒蛋白/RC 3，甲状腺激素和类维生素A的转录激活的目标。这些研究结果表明，PR诱导的小鼠脑神经元的保护涉及甲状腺激素和/或维甲酸和增强糖酵解引起的神经营养事件。(C)2008年爱思唯尔公司All rights reserved.
Cobalt focus is a seizure focus model in which cerebral neurons exhibit long-lasting severe spike discharges, followed by neuronal death. However, the neuronal death is prevented when peony root extract (PR) is administered prior to cobalt application. We tested the hypothesis that PR modulates the expression of neuroprotective proteins in the cerebrum of mouse cobalt focus by proteomic analysis using two-dimensional polyacrylamide gel electrophoresis and mass spectrometry to screen for differentially expressed proteins. Analyses revealed that transthyretin, a carrier protein for thyroid hormones and retinoids, and the brain form of phosphoglycerate mutase, a glycolytic enzyme, were upregulated in the cobalt-treated mouse cerebrum and further increased by PR administration in association with upregulation of neurogranin/RC3, a target of the transcriptional activation by thyroid hormones and retinoids. These findings suggest that PR-induced protection of mouse cerebral neurons involves neurotrophic events caused by thyroid hormones and/or retinoids and enhanced glycolysis. (C) 2008 Elsevier Inc. All rights reserved.