PHOTODYNAMIC THERAPY USING A DIODE-LASER WITH MONO-L-ASPARTYL CHLORIN E6 FOR IMPLANTED FIBROSARCOMA IN MICE
PHOTODYNAMIC THERAPY USING A DIODE-LASER WITH MONO-L-ASPARTYL CHLORIN E6 FOR IMPLANTED FIBROSARCOMA IN MICE
复制标题
DOI：
10.1111/j.1349-7006.1994.tb02923.x
复制
发表时间：
1994-11-01
期刊：
影响因子：
--
通讯作者：
KATO, H
中科院分区：
文献类型：
--
作者：
KATSUMI, T;AIZAWA, K;KATO, H
我们已经开发了一种新的高功率红色（664 nm）激光二极管系统的光动力学治疗（PDT）与单-L-乙酰二氢卟酚e6（NPe 6）。将Meth-A纤维肉瘤细胞（1 × 10（6））皮下植入4周龄BALB/c雌性小鼠的右后腿。一周后，在对每只荷瘤小鼠静脉内施用NPe 6后5小时施加二极管激光照射。在第一项研究中，PDT期间肿瘤内温度升高的时间过程通过使用23号热电偶皮下注射针在距离肿瘤表面2 mm的深度处测量。在第二项研究中，在以1.25、2.5、5.0或7.5 mg/kg/小鼠的剂量静脉内施用NPe 6后5小时，用二极管激光器处理6组10至17只荷瘤小鼠。总光照射范围为0至150 J/cm（2），剂量率调整为100 mW/cm（2）。治愈率由治疗后50天明显无病的小鼠数量确定。结果表明，这种半导体激光器是有效的PDT植入纤维肉瘤后，NPe 6管理。这也证实了PDT的治疗效果不是由于热疗。此外，通过CCD技术证明了二极管激光束在整个光照射场中强度均匀。
We have developed a new high-power red (664 nm) laser diode system for photodynamic therapy (PDT) with mono-L-aspartyl chlorin e6 (NPe6). Meth-A fibrosarcoma cells (1X10(6)) were implanted subcutaneously in the right hind leg of 4-week-old BALB/c female mice. One week later, diode laser irradiation was applied 5 h after the intravenous administration of NPe6 to each tumor-bearing mouse. In the first study, the time course of intratumor temperature increase during PDT was measured by using a 23-guage thermocouple hypodermic needle at a depth of 2 mm from the tumor surface. In the second study, 6 groups of 10 to 17 tumor-bearing mice were treated with the diode laser 5 h after intravenous administration of NPe6 at the dose of 1.25, 2.5, 5.0 or 7.5 mg/kg i.v. per mouse. Total photoirradiation ranged from 0 to 150 J/cm(2) and the dose rate was adjusted to 100 mW/cm(2). Percentages of cures were determined from numbers of mice apparently disease-free 50 days after treatment. The results showed that this diode laser is effective in PDT of implanted fibrosarcoma after NPe6 administration. It also confirmed that the therapeutic effects of PDT were not due to hyperthermia. Moreover, the diode laser beam was demonstrated by CCD technology to be uniform in intensity throughout the photoirradiated field.