Cell-free production of functional Plasmodium falciparum dihydrofolate reductase-thymidylate synthase
Cell-free production of functional Plasmodium falciparum dihydrofolate reductase-thymidylate synthase
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DOI：
10.1016/j.molbiopara.2006.10.016
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发表时间：
2007-02-01
影响因子：
1.5
通讯作者：
Rathod, Pradipsinh K.
中科院分区：
文献类型：
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作者：
Mudeppa, Devaraja G.;Pang, Cullen K. T.;Rathod, Pradipsinh K.
本文证明了利用小麦胚芽翻译系统可以清洁地生产疟疾蛋白二氢叶酸还原酶-胸苷合成酶。除了这两种催化功能外，专性同源二聚体还具有自身的RNA结合功能。这些发现为表达功能性P提供了新的机会。恶性疟原虫蛋白。
This article demonstrates that a malaria protein, dihydrofolate reductase-thymidylate synthase, can be produced cleanly using a wheat germ translation system. In addition to the two catalytic functions, the obligate homodimer also carries an autologous RNA-binding function. These findings point to new opportunity for expressing functionalP. falciparumproteins.