Lack of inducibility of brain monooxygenase activities including parathion desulfuration.
Lack of inducibility of brain monooxygenase activities including parathion desulfuration.
复制标题
缺乏脑单加氧酶活性的诱导性，包括对硫磷脱硫。
DOI：
10.1002/jbt.2570040111
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Forsyth,CS
中科院分区：
文献类型：
--
作者：
Chambers,JE;Forsyth,CS
本文研究了苯巴比妥和β-萘黄酮对雄性和雌性大鼠脑和肝脏中对硫磷的脱除、氨基比林N-脱甲基化和NADPH-细胞色素-还原酶活性的影响。这三种酶的活性在大脑的线粒体和微粒体部分中都发现了相似的水平。大脑活动没有性别差异。当按组织湿重当量计算时，肝脏活动比大脑活动高10到30倍。尽管暴露于两种诱导剂都能增加肝脏中三种酶的活性和细胞色素P-450，但这两种诱导剂都不会增加两性线粒体或微米体脑部分的酶活性。因此，这些脑单加氧酶活性似乎不能被两种经典类型的细胞色素P-450诱导剂诱导。这种缺乏诱导性可以用来保护动物免受环境增强的外源物质对神经毒性代谢物的激活增加，例如对硫磷转化为对氧磷。
The ability of phenobarbital and β‐naphthoflavone to induce parathion desulfuration, aminopyrineN‐demethylation, and NADPH–cyto‐chrome‐creductase activity in the brain and liver of male and female rats was investigated. Activities of all three enzymes were found in similar levels in both the mitochondrial and microsomal fractions of brain. There were no sex differences in brain activities. Liver activities were from 10‐ to 30‐fold higher than brain activities when computed on a tissue‐wet‐weight‐equivalent basis. Although exposure to both inducers increased all three enzyme activities and cytochromeP‐450 in liver, neither inducer increased the enzyme activities in mitochondrial or microsomal brain fractions of either sex. Thus, these brain monooxygenase activities appear to be refractory to induction by two classical types of cytochromeP‐450 inducers. This lack of inducibility could serve to protect the animal against environmentally enhanced increases in the activation of xenobiotics to neurotoxic metabolites, such as parathion desulfuration to paraoxon.