Replicative retroviral vectors for cancer gene therapy
Replicative retroviral vectors for cancer gene therapy
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DOI：
10.1038/sj.cgt.7700521
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发表时间：
2003-01-01
影响因子：
6.4
通讯作者：
Klatzmann, D
中科院分区：
文献类型：
--
作者：
Solly, SK;Trajcevski, S;Klatzmann, D
基因转移到癌细胞的效率低下是当前癌症基因治疗的主要瓶颈。我们推断，由于肿瘤是大量快速分裂的细胞，因此使用允许转基因传播的载体系统可以最有效地转导它们。因此，我们设计了两种复制性逆转录病毒衍生载体系统：一种固有复制载体，一种由辅助逆转录病毒繁殖的缺陷载体。在体外，两个系统都实现了非常有效的转基因繁殖。在免疫功能正常的小鼠中，复制型载体转导了 >85% 的肿瘤细胞，而有缺陷的载体则转导了
Poor efficiency of gene transfer into cancer cells constitutes the major bottleneck of current cancer gene therapy. We reasoned that because tumors are masses of rapidly dividing cells, they would be most efficiently transduced with vector systems allowing transgene propagation. We thus designed two replicative retrovirus-derived vector systems: one inherently replicative vector, and one defective vector propagated by a helper retrovirus. In vitro, both systems achieved very efficient transgene propagation. In immunocompetent mice, replicative vectors transduced >85% tumor cells, whereas defective vectors transduced